ArticleJournal of clinical microbiology2008
Comparison of PCR, immunofluorescence assay, and pathogen isolation for diagnosis of q fever in humans with spontaneous abortions.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01095328 (Cost-effectiveness of a Screening Strategy for Q Fever Among Pregnant Women in Risk Areas), which is not on this map. Cited by 36 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Cost-effectiveness of a Screening Strategy for Q Fever Among Pregnant Women in Risk Areas: a Clustered Randomized Controlled Trial
Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it, 86 citations in OpenAlex.
- Prevalence and Risk Factors of Coxiellosis at the Human-Animal-Environment Interface in the South Asian Countries: A Systematic Review and Meta-Analysis.Transboundary and emerging diseases · 2025Pooled it
- Occupational Zoonotic Risk at India's Human-Animal Interface: Statutory Origins and a Regulatory Pathway for the Compensation-Prevention Disconnect.American journal of industrial medicine · 2026Article
- Sources and culture methods for the isolation and cultivation ofFrontiers in microbiology · 2026Review
- Evidence ofNew microbes and new infections · 2025Review
- Clinical diagnosis of Q fever by targeted next-generation sequencing for identification of Coxiella burnetii.BMC infectious diseases · 2025Article
- Can Wastewater Surveillance Enhance Genomic Tracking of Climate-Driven Pathogens?Microorganisms · 2025Review
- Molecular characterisation ofFrontiers in veterinary science · 2025Article
- Laboratory investigation of causes of bovine abortion and stillbirth in the Republic of Korea, 2014-2020.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2024Article
- Assessing and Prioritizing Zoonotic Diseases in Punjab, India: A One Health Approach.EcoHealth · 2023Article
- Article
- A Microfluidic In Vitro Three-Dimensional Dynamic Model of the Blood-Brain Barrier to Study the Transmigration of Immune Cells.Brain sciences · 2022Article
- Shedding and extensive and prolonged environmental contamination of goat farms of Q fever patients by Coxiella burnetii.Veterinary medicine and science · 2022Article
- Diagnosis ofPathogens (Basel, Switzerland) · 2022Article
- Real-time PCR biochip for on-site detection of Coxiella burnetii in ticks.Parasites & vectors · 2021Article
- High prevalence ofVeterinary world · 2020Article
- Seroprevalence and Molecular Characterization ofPathogens (Basel, Switzerland) · 2020Article
- Q fever: A neglected disease of camels in Giza and Cairo Provinces, Egypt.Veterinary world · 2019Article
- Article
- Q Fever in the First Trimester: A Case Report from Northern Rural New South Wales.Tropical medicine and infectious disease · 2019Article
- Detection of the presence of Coxiella burnetii in a case of goat abortion: a first report from India.Tropical animal health and production · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coxiella burnetii, an obligate intracellular parasite with a worldwide distribution, is the causative agent of Q fever in humans. We tested a total of 368 samples (placental bits, genital swabs, fecal swabs, and urine and serum samples) collected from women (n = 74) with spontaneous abortions for C. burnetii by a PCR assay targeting IS1111, the repetitive transposon-like region of C. burnetii (trans-PCR); real-time PCR; an indirect immunofluorescence assay (IFA); and the isolation of the pathogen. The IFA showed seropositivity for 25.68% of the women with spontaneous abortions, whereas trans-PCR and real-time PCR each detected the pathogen in 21.62% of cases. Overall, 25.68% of the subjects were positive by one or more assays. Real-time PCR showed a slightly higher level of sensitivity than trans-PCR. With the IFA as the reference, the two PCR assays showed a higher level of sensitivity (84.21%) than pathogen isolation (26.31%), while both the PCR assays and pathogen isolation were specific (100%). The detection of high numbers of C. burnetii cells in clinical samples and the frequent association of the pathogen with cases of spontaneous abortions observed in this study revealed that Q fever remains underdiagnosed and that the prevalence in India is underestimated.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.