ArticleMolecular biology of the cell2008
Nucleophosmin is a binding partner of nucleostemin in human osteosarcoma cells.
Article in Molecular biology of the cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 28 citations in OpenAlex.
- Article
- Article
- Proteomic Investigation of the Role of Nucleostemin in Nucleophosmin-Mutated OCI-AML 3 Cell Line.International journal of molecular sciences · 2022Article
- Mobility of Nucleostemin in Live Cells Is Specifically Related to Transcription Inhibition by Actinomycin D and GTP-Binding Motif.International journal of molecular sciences · 2021Article
- Tumor suppressor PNRC1 blocks rRNA maturation by recruiting the decapping complex to the nucleolus.The EMBO journal · 2018Article
- FAK activity protects nucleostemin in facilitating breast cancer spheroid and tumor growth.Breast cancer research : BCR · 2015Article
- Article
- The nucleolus stress response is coupled to an ATR-Chk1-mediated G2 arrest.Molecular biology of the cell · 2013Article
- Nucleolar protein GLTSCR2 stabilizes p53 in response to ribosomal stresses.Cell death and differentiation · 2012Article
- Nucleolar stress is an early response to myocardial damage involving nucleolar proteins nucleostemin and nucleophosmin.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- The nucleolus.Cold Spring Harbor perspectives in biology · 2011Review
- Self-Renewal Signalling in Presenescent Tetraploid IMR90 Cells.Journal of aging research · 2011Article
- NPM1/B23: A Multifunctional Chaperone in Ribosome Biogenesis and Chromatin Remodeling.Biochemistry research international · 2011Article
- Protein B23/nucleophosmin/numatrin nuclear dynamics in relation to protein kinase CK2 and apoptotic activity in prostate cells.Biochemistry · 2010Article
- The Nucleolus Takes Control of Protein Trafficking Under Cellular Stress.Molecular and cellular pharmacology · 2010Article
- Knockdown of the Drosophila GTPase nucleostemin 1 impairs large ribosomal subunit biogenesis, cell growth, and midgut precursor cell maintenance.Molecular biology of the cell · 2009Article
- Novel role of nucleostemin in the maintenance of nucleolar architecture and integrity of small nucleolar ribonucleoproteins and the telomerase complex.The Journal of biological chemistry · 2009Article
- MicroRNAs with a nucleolar location.RNA (New York, N.Y.) · 2009Article
- Genetic ablation of caveolin-1 drives estrogen-hypersensitivity and the development of DCIS-like mammary lesions.The American journal of pathology · 2009Article
- In search of nonribosomal nucleolar protein function and regulation.The Journal of cell biology · 2009Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucleostemin (NS) is expressed in the nucleoli of adult and embryonic stem cells and in many tumors and tumor-derived cell lines. In coimmunoprecipitation experiments, nucleostemin is recovered with the tumor suppressor p53, and more recently we have demonstrated that nucleostemin exerts its role in cell cycle progression via a p53-dependent pathway. Here, we report that in human osteosarcoma cells, nucleostemin interacts with nucleophosmin, a nucleolar protein believed to possess oncogenic potential. Nucleostemin (NS) and nucleophosmin (NPM) displayed an extremely high degree of colocalization in the granular component of the nucleolus during interphase, and both proteins associated with prenucleolar bodies in late mitosis before the reformation of nucleoli. Coimmunoprecipitation experiments revealed that NS and NPM co-reside in complexes, and yeast two-hybrid experiments confirmed that they are interactive proteins, revealing the NPM-interactive region to be the 46-amino acid N-terminal domain of NS. In bimolecular fluorescence complementation studies, bright nucleolar signals were observed, indicating that these two proteins directly interact in the nucleolus in vivo. These results support the notion that cell cycle regulatory proteins congress and interact in the nucleolus, adding to the emerging concept that this nuclear domain has functions beyond ribosome production.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.