ArticleBMC ophthalmology2008
Specific detection of fungal pathogens by 18S rRNA gene PCR in microbial keratitis.
Article in BMC ophthalmology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 89 citations in OpenAlex.
- Metabarcoding insights into the fungal diversity and biotechnological potential of mangrove sediments in Ecuador's Reserva Ecológica Manglares Churute.Frontiers in fungal biology · 2026Article
- Causative Agents of Fungal Keratitis in Northeastern Iran: A 3-Year Tertiary Care Hospital Study.The American journal of tropical medicine and hygiene · 2025Article
- Iron modulation drives biofilm formation and virulence enzyme production in emerging clinicalFrontiers in fungal biology · 2025Article
- Diagnostic Performance of a Molecular Assay in Synovial Fluid Targeting Dominant Prosthetic Joint Infection Pathogens.Microorganisms · 2024Article
- Recent Advances in Diagnosis and Treatment Approaches in Fungal Keratitis: A Narrative Review.Microorganisms · 2024Review
- Microbiological culture versus 16S/18S rRNA gene PCR-sanger sequencing for infectious keratitis: a three-arm, diagnostic cross-sectional study.Frontiers in medicine · 2024Article
- Radiometal chelators for infection diagnostics.Frontiers in nuclear medicine · 2023Article
- Morphological and Molecular Variability ofInternational journal of microbiology · 2023Review
- Diagnostic methods for the etiological assessment of infectious corneal pathology (Review).Experimental and therapeutic medicine · 2022Review
- Recent Perspectives in the Management of Fungal Keratitis.Journal of fungi (Basel, Switzerland) · 2021Review
- Practical Guidance for Clinical Microbiology Laboratories: Diagnosis of Ocular Infections.Clinical microbiology reviews · 2021Review
- Identification and molecular characterization of Subramaniula asteroides causing human fungal keratitis: a case report.BMC infectious diseases · 2021Article
- Filamentous Fungal Keratitis in Taiwan: Based on Molecular Diagnosis.Translational vision science & technology · 2020Article
- An Omics Approach to Diagnosing or Investigating Fungal Keratitis.International journal of molecular sciences · 2019Review
- Fungal Keratitis: Epidemiology, Rapid Detection, and Antifungal Susceptibilities ofBioMed research international · 2019Article
- Diagnosis of Superficial Mycoses by a Rapid and Effective PCR Method from Samples of Scales, Nails and Hair.Mycopathologia · 2018Article
- Detection of fungi by conventional methods and semi-nested PCR in patients with presumed fungal keratitis.Current medical mycology · 2015Article
- Autochthonous blastomycosis of the adrenal: first case report from Asia.The American journal of tropical medicine and hygiene · 2014Article
- Role of molecular diagnostics in ocular microbiology.Current ophthalmology reports · 2013Article
- Diagnosis of infectious diseases of the eye.Eye (London, England) · 2012Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe sensitivity and specificity of 18S rRNA polymerase chain reaction (PCR) in the detection of fungal aetiology of microbial keratitis was determined in thirty patients with clinical diagnosis of microbial keratitis.
methodsCorneal scrapings from patients were used for Gram stain, culture and PCR analysis. PCR was performed with primer pairs targeted to the 18S rRNA gene. The result of the PCR was compared with conventional culture and Gram staining method. The PCR positive samples were identified by DNA sequencing of the internal transcribed spacer (ITS) region of the rRNA gene. Main outcome measures were sensitivity and specificity of PCR in the detection of fungus in corneal keratitis.
resultsCombination of microscopy and culture gave a positive result in 11 of 30 samples of microbial keratitis. PCR detected 10 of 11 samples that were positive by conventional method. One of the 19 samples that was negative by conventional method was positive by PCR. Statistical analysis revealed that the PCR to have a sensitivity of 90.9% and specificity of 94.7% in the detection of a fungal aetiology in microbial keratitis.
conclusionPCR is a rapid, sensitive and useful method to detect fungal aetiology in microbial keratitis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.