ArticleThe Journal of general physiology2008
IP3 sensitizes TRPV4 channel to the mechano- and osmotransducing messenger 5'-6'-epoxyeicosatrienoic acid.
Article in The Journal of general physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Inhibition of intracellular proton-sensitive CaActa pharmaceutica Sinica. B · 2022Article
- The role of TRPV4 channels in ocular function and pathologies.Experimental eye research · 2020Review
- Channels that Cooperate with TRPV4 in the Brain.Journal of molecular neuroscience : MN · 2020Review
- Steroids and TRP Channels: A Close Relationship.International journal of molecular sciences · 2020Review
- Volume sensing in the transient receptor potential vanilloid 4 ion channel is cell type-specific and mediated by an N-terminal volume-sensing domain.The Journal of biological chemistry · 2019Article
- Dual contribution of TRPV4 antagonism in the regulatory effect of vasoinhibins on blood-retinal barrier permeability: diabetic milieu makes a difference.Scientific reports · 2017Article
- When size matters: transient receptor potential vanilloid 4 channel as a volume-sensor rather than an osmo-sensor.The Journal of physiology · 2017Article
- Beyond neurovascular coupling, role of astrocytes in the regulation of vascular tone.Neuroscience · 2016Review
- How does rhinovirus cause the common cold cough?BMJ open respiratory research · 2016Article
- Endothelium-Derived Hyperpolarization and Coronary Vasodilation: Diverse and Integrated Roles of Epoxyeicosatrienoic Acids, Hydrogen Peroxide, and Gap Junctions.Microcirculation (New York, N.Y. : 1994) · 2016Review
- Phosphorylation on TRPV4 Serine 824 Regulates Interaction with STIM1.The open biochemistry journal · 2015Article
- Endothelial control of vasodilation: integration of myoendothelial microdomain signalling and modulation by epoxyeicosatrienoic acids.Pflugers Archiv : European journal of physiology · 2014Review
- TRPV4 in porcine lens epithelium regulates hemichannel-mediated ATP release and Na-K-ATPase activity.American journal of physiology. Cell physiology · 2012Article
- International Union of Basic and Clinical Pharmacology. LXXVI. Current progress in the mammalian TRP ion channel family.Pharmacological reviews · 2010Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mechanical and osmotic sensitivity of the transient receptor potential vanilloid 4 (TRPV4) channel depends on phospholipase A2 (PLA2) activation and the subsequent production of the arachidonic acid metabolites, epoxyeicosatrienoic acid (EET). We show that both high viscous loading and hypotonicity stimuli in native ciliated epithelial cells use PLA2-EET as the primary pathway to activate TRPV4. Under conditions of low PLA2 activation, both also use extracellular ATP-mediated activation of phospholipase C (PLC)-inositol trisphosphate (IP3) signaling to support TRPV4 gating. IP3, without being an agonist itself, sensitizes TRPV4 to EET in epithelial ciliated cells and cells heterologously expressing TRPV4, an effect inhibited by the IP3 receptor antagonist xestospongin C. Coimmunoprecipitation assays indicated a physical interaction between TRPV4 and IP3 receptor 3. Collectively, our study suggests a functional coupling between plasma membrane TRPV4 channels and intracellular store Ca2+ channels required to initiate and maintain the oscillatory Ca2+ signal triggered by high viscosity and hypotonic stimuli that do not reach a threshold level of PLA2 activation.
Identifiers
18443356What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.