ArticleInfection and immunity2008
Pathogenesis of renal disease due to enterohemorrhagic Escherichia coli in germ-free mice.
Article in Infection and immunity, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
51 citing papers in PubMed, 76 citations in OpenAlex.
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- Pathogenomes of Shiga Toxin Positive and NegativeMicroorganisms · 2024Article
- Efficacy of EHEC gold nanoparticle vaccines evaluated with the Shiga toxin-producingMicrobiology spectrum · 2024Article
- CommensalAnimals : an open access journal from MDPI · 2023Article
- Pioneer colonizers: Bacteria that alter the chicken intestinal morphology and development of the microbiota.Frontiers in physiology · 2023Article
- Pathogenomes and variations in Shiga toxin production among geographically distinct clones ofMicrobial genomics · 2022Article
- Mcc1229, an Stx2a-Amplifying Microcin, Is ProducedInfection and immunity · 2022Article
- Citrobacter rodentium(ϕStx2dact), a murine infection model for enterohemorrhagic Escherichia coli.Current opinion in microbiology · 2022Review
- Nosocomial Infections and Role of Nanotechnology.Bioengineering (Basel, Switzerland) · 2022Review
- Stx2 Induces Differential Gene Expression and Disturbs Circadian Rhythm Genes in the Proximal Tubule.Toxins · 2022Article
- EntericFrontiers in veterinary science · 2022Review
- Review
- A One Health Perspective for Defining and DecipheringComparative medicine · 2021Review
- Citrobacter rodentium Lysogenized with a Shiga Toxin-Producing Phage: A Murine Model for Shiga Toxin-Producing E. coli Infection.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Interplay between enterohaemorrhagicEmerging microbes & infections · 2020Article
- Prevention of enteric bacterial infections and modulation of gut microbiota with conjugated linoleic acids producingGut microbes · 2020Article
- Pathogenomes of Atypical Non-shigatoxigenicFrontiers in microbiology · 2020Article
- A Rare Opportunist,Infection and immunity · 2019Article
- Protective effects of bifidobacteria against enteropathogens.Microbial biotechnology · 2019Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Enterohemorrhagic Escherichia coli (EHEC) is a food-borne pathogen that causes hemorrhagic colitis and acute renal failure. We used a germ-free mouse model to investigate the role of host factors, Shiga toxin 2 (Stx2), and bacterial strain in disease due to EHEC. Germ-free male and female Swiss-Webster mice that were 3 days to 12 weeks old were orally inoculated with 1 of 10 EHEC strains or derivatives of two of these strains with Stx2 deleted. All inoculated mice became infected regardless of the inoculum dose. All bacterial strains colonized the intestines, reaching levels of 10(9) to 10(12) CFU/g of feces by 4 days after inoculation. Seven of the 10 wild-type strains caused disease. However, the two Stx2 deletion mutants, unlike the Stx2(+) parental strains, did not cause disease. The clinical signs of disease in mice included lethargy, dehydration, polyuria, polydypsia, and death. Postmortem examination of affected mice revealed dehydration and luminal cecal fluid accumulation. Histologic examination revealed close adherence of bacteria to the intestinal epithelium in the ileum and cecum but not in the colon. Other lesions included progressive renal tubular necrosis, glomerular fibrin thrombosis, and red blood cell sludging. The severity of disease varied according to the bacterial strain and age, but not sex, of the host. This study demonstrated that EHEC colonizes germ-free mice in large numbers, adheres to the intestinal epithelium, and causes luminal cecal fluid accumulation and progressive renal failure. The disease in mice was Stx2 and bacterial strain dependent. This animal model should be a useful tool for studying the pathogenesis of renal disease secondary to EHEC infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.