ArticlePlant methods2008
High quality metabolomic data for Chlamydomonas reinhardtii.
Article in Plant methods, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed.
- Metabolomic Profiling ofJournal of fungi (Basel, Switzerland) · 2026Article
- Comparative Metabolomics ofJournal of microbiology and biotechnology · 2025Article
- Ninjurin 2 Modulates Tumorigenesis, Inflammation, and Metabolism via Pyroptosis.The American journal of pathology · 2024Article
- Rootstock and Crop Load Effects on 'Honeycrisp' Photosynthetic Performance and Carbohydrate Accumulation.Plants (Basel, Switzerland) · 2023Article
- Pathogen-triggered metabolic adjustments to potato virus Y infection in potato.Frontiers in plant science · 2022Article
- Root Bark ofJournal of microbiology and biotechnology · 2021Article
- Root Exudates Alter the Expression of Diverse Metabolic, Transport, Regulatory, and Stress Response Genes in RhizosphereFrontiers in microbiology · 2021Article
- Metabolomic Elucidation of the Effect of Sucrose on the Secondary Metabolite Profiles inACS omega · 2020Article
- Organic Farming Sharpens Plant Defenses in the Field.Frontiers in sustainable food systems · 2020Article
- Controlled replication of 'Candidatus Liberibacter asiaticus' DNA in citrus leaf discs.Microbial biotechnology · 2020Article
- Metabolomic Investigation on Fermentation Products ofJournal of microbiology and biotechnology · 2020Article
- An Ancient Residue Metabolomics-Based Method to Distinguish Use of Closely Related Plant Species in Ancient Pipes.Frontiers in molecular biosciences · 2020Article
- Systematic re-evaluation of the long-used standard protocol of urease-dependent metabolome sample preparation.PloS one · 2020Article
- Development and characterization of aBiotechnology for biofuels · 2020Article
- Posttranslational Modification of the NADP-Malic Enzyme Involved in CThe Plant cell · 2019Article
- Improved Algal Toxicity Test System for RobustMetabolites · 2019Article
- Metabolomic and Transcriptomic Analyses ofMarine drugs · 2019Article
- Article
- Suboptimal Temperature Acclimation Affects Kennedy Pathway Gene Expression, Lipidome and Metabolite Profile ofMarine drugs · 2018Article
- Quenching for Microalgal Metabolomics: A Case Study on the Unicellular Eukaryotic Green AlgaMetabolites · 2018Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The green eukaryote alga Chlamydomonas reinhardtii is a unicellular model to study control of metabolism in a photosynthetic organism. We here present method improvements for metabolite profiling based on GC-TOF mass spectrometry focusing on three parameters: quenching and cell disruption, extract solvent composition and metabolite annotation. These improvements facilitate using smaller cell numbers and hence, smaller culture volumes which enable faster and more precise sampling techniques that eventually lead to a higher number of samples that can be processed, e.g. for time course experiments. Quenching of metabolism was achieved by mixing 1 ml of culture to 1 ml of -70 degrees C cold 70% methanol. After centrifugation, cells were lyophilized and disrupted by milling using 2-6E6 lyophilized cells, around 500-fold less than previously reported. Glass beads were compared to metal balls for milling, and five different extraction solvents were tested. Additionally, all peaks were annotated in an automated way using the GC-TOF database BinBase instead of manual investigation of a single reference chromatogram. Median precision of analysis was used to decide for the eventual procedure which was applied to a proof-of-principle study of time dependent changes of metabolism under standard conditions.
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Registered trials
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