ArticlePlant physiology2008
Functional identification of Arabidopsis stress regulatory genes using the controlled cDNA overexpression system.
Article in Plant physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 139 citations in OpenAlex.
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- Limited directional selection but coevolutionary signals among imprinted genes inbioRxiv : the preprint server for biology · 2026Article
- Discrete and cell-specific hypoxic responses in Arabidopsis roots resolved by single-nuclei transcriptomics.The New phytologist · 2026Article
- Plastid-to-nucleus communication under hypoxia involves group VII ethylene response factors inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Roles of ROS and NO in Plant Responses to Individual and Combined Salt Stress and Waterlogging.Antioxidants (Basel, Switzerland) · 2025Review
- Large-Scale Rice Mutant Establishment and High-Throughput Mutant Manipulation Help Advance Rice Functional Genomics.Plants (Basel, Switzerland) · 2025Review
- Modification of xylan in secondary walls alters cell wall biosynthesis and wood formation programs and improves saccharification.Plant biotechnology journal · 2025Article
- Recent progress in understanding the cellular and genetic basis of plant responses to low oxygen holds promise for developing flood-resilient crops.Journal of experimental botany · 2024Article
- High-gradient magnetic fields and starch metabolism: results from a space experiment.Scientific reports · 2022Article
- A Molecular Signal Integration Network Underpinning Arabidopsis Seed Germination.Current biology : CB · 2020Article
- The alcohol dehydrogenase gene family in sugarcane and its involvement in cold stress regulation.BMC genomics · 2020Article
- Compensation of Mutation inInternational journal of molecular sciences · 2020Article
- Strigolactones Interact With Nitric Oxide in Regulating Root System Architecture ofFrontiers in plant science · 2020Article
- Reactive Carbonyl Species: A Missing Link in ROS Signaling.Plants (Basel, Switzerland) · 2019Review
- The mitogen-activated protein kinase 4-phosphorylated heat shock factor A4A regulates responses to combined salt and heat stresses.Journal of experimental botany · 2019Article
- Nitrate nutrition influences multiple factors in order to increase energy efficiency under hypoxia in Arabidopsis.Annals of botany · 2019Article
- Progress toward Understanding the Molecular Basis of Fruit Response to Hypoxia.Plants (Basel, Switzerland) · 2018Review
- Development of iFOX-hunting as a functional genomic tool and demonstration of its use to identify early senescence-related genes in the polyploid Brassica napus.Plant biotechnology journal · 2018Article
- Plant cysteine oxidases are dioxygenases that directly enable arginyl transferase-catalysed arginylation of N-end rule targets.Nature communications · 2017Article
- Jasmonate Regulates Plant Responses to Postsubmergence Reoxygenation through Transcriptional Activation of Antioxidant Synthesis.Plant physiology · 2017Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Responses to environmental stresses in higher plants are controlled by a complex web of abscisic acid (ABA)-dependent and independent signaling pathways. To perform genetic screens for identification of novel Arabidopsis (Arabidopsis thaliana) loci involved in the control of abiotic stress responses, a complementary DNA (cDNA) expression library was created in a Gateway version of estradiol-inducible XVE binary vector (controlled cDNA overexpression system [COS]). The COS system was tested in three genetic screens by selecting for ABA insensitivity, salt tolerance, and activation of a stress-responsive ADH1-LUC (alcohol dehydrogenase-luciferase) reporter gene. Twenty-seven cDNAs conferring dominant, estradiol-dependent stress tolerance phenotype, were identified by polymerase chain reaction amplification and sequence analysis. Several cDNAs were recloned into the XVE vector and transformed recurrently into Arabidopsis, to confirm that the observed conditional phenotypes were due to their estradiol-dependent expression. Characterization of a cDNA conferring insensitivity to ABA in germination assays has identified the coding region of heat shock protein HSP17.6A suggesting its implication in ABA signal transduction. Screening for enhanced salt tolerance in germination and seedling growth assays revealed that estradiol-controlled overexpression of a 2-alkenal reductase cDNA confers considerable level of salt insensitivity. Screening for transcriptional activation of stress- and ABA-inducible ADH1-LUC reporter gene has identified the ERF/AP2-type transcription factor RAP2.12, which sustained high-level ADH1-LUC bioluminescence, enhanced ADH1 transcription rate, and increased ADH enzyme activity in the presence of estradiol. These data illustrate that application of the COS cDNA expression library provides an efficient strategy for genetic identification and characterization of novel regulators of abiotic stress responses.
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