ArticleJournal of bacteriology2008
A genomic islet mediates flagellar phase variation in Escherichia coli strains carrying the flagellin-specifying locus flk.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Bioinformatics Analysis and Spatiotemporal Distribution of the fliC Gene and Its Protein Isolated from Escherichia coli-Infected Patients in Eastern Algeria.The Malaysian journal of medical sciences : MJMS · 2024Article
- Static Magnetic Field Inhibits Growth ofCells · 2022Article
- Diversification ofFrontiers in microbiology · 2021Article
- Flagellum-Mediated Mechanosensing and RflP Control Motility State of Pathogenic Escherichia coli.mBio · 2020Article
- Article
- Comparative genomic analysis of the secondary flagellar (flag-2) system in the order Enterobacterales.BMC genomics · 2020Article
- A Targeted Sequencing Assay for SerotypingFrontiers in microbiology · 2020Article
- Escherichia coli H-Genotyping PCR: a Complete and Practical Platform for Molecular H Typing.Journal of clinical microbiology · 2018Article
- No evidence for a bovine mastitis Escherichia coli pathotype.BMC genomics · 2017Article
- Comparative genomic analysis of the flagellin glycosylation island of the Gram-positive thermophile Geobacillus.BMC genomics · 2016Article
- Hybrid Shiga Toxin-Producing and Enterotoxigenic Escherichia sp. Cryptic Lineage 1 Strain 7v Harbors a Hybrid Plasmid.Applied and environmental microbiology · 2016Article
- Article
- Rapid and Easy In Silico Serotyping of Escherichia coli Isolates by Use of Whole-Genome Sequencing Data.Journal of clinical microbiology · 2015Article
- A novel non-homologous recombination-mediated mechanism for Escherichia coli unilateral flagellar phase variation.Nucleic acids research · 2012Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
Abstract
The occurrence of unilateral flagellar phase variation was previously demonstrated in Escherichia coli strains carrying the non-fliC flagellin-specifying locus flk. In this study, we investigated the mechanism involved in this process. By using sequencing and sequence analysis, the flk region between the chromosomal genes yhaC and rnpB was characterized in all described flk-positive E. coli strains, including the H35 strain identified in this study (the other strains used are H3, H36, H47, and H53 strains), and this region was found to contain a putative integrase gene and flanking direct repeats in addition to the flk flagellin-specifying gene flkA and a fliC repressor gene, flkB, indicating that there is a typical genomic islet (GI), which was designated the flk GI. The horizontal transfer potential of the flk GI was indicated by detection of the excised extrachromosomal circular form of the flk GI. By generating fliC-expressing variants of H3 and H47 strains, unilateral flagellar phase variation in flk-positive strains was shown to be mediated by excision of the flk GI. The function of the proposed integrase gene was confirmed by deletion and a complementation test. The potential integration sites of the flk GI were identified. A general model for flagellar phase variation in flk-positive E. coli strains can be expressed as fliC(off) + flkA(on) --> fliC(on) + flkA(none). This is the first time that a molecular mechanism for flagellar phase variation has been reported for E. coli.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.