ArticleJournal of bacteriology2008
Glucomannan-mediated attachment of Rhizobium leguminosarum to pea root hairs is required for competitive nodule infection.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
58 citing papers in PubMed, 124 citations in OpenAlex.
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- Unlocking the potential of biofilm-forming plant growth-promoting rhizobacteria for growth and yield enhancement in wheat (Triticum aestivum L.).Scientific reports · 2024Article
- Exploring the cellular surface polysaccharide and root nodule symbiosis characteristics of theMicrobiology spectrum · 2023Article
- Dual adhesive unipolar polysaccharides synthesized by overlapping biosynthetic pathways in Agrobacterium tumefaciens.Molecular microbiology · 2022Article
- Plant-microbe interactions in the apoplast: Communication at the plant cell wall.The Plant cell · 2022Article
- Spatial organization of the kelp microbiome at micron scales.Microbiome · 2022Article
- RapD Is a Multimeric Calcium-Binding Protein That Interacts With theFrontiers in microbiology · 2022Article
- Deciphering bacterial mechanisms of root colonization.Environmental microbiology reports · 2021Review
- Structure and Development of the Legume-Rhizobial Symbiotic Interface in Infection Threads.Cells · 2021Review
- Exopolysaccharide Carbohydrate Structure and Biofilm Formation byInternational journal of molecular sciences · 2021Article
- Competition, Nodule Occupancy, and Persistence of Inoculant Strains: Key Factors in theFrontiers in plant science · 2021Review
- Pectin Induced Colony Expansion of Soil-DerivedFrontiers in microbiology · 2021Article
- Biofilm Producing Rhizobacteria With Multiple Plant Growth-Promoting Traits Promote Growth of Tomato Under Water-Deficit Stress.Frontiers in microbiology · 2020Article
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- Composition of the Holdfast Polysaccharide fromJournal of bacteriology · 2019Article
- Transcriptomic Studies Reveal that theInternational journal of molecular sciences · 2019Article
- Biofilm-Constructing Variants of Paraburkholderia phytofirmans PsJN Outcompete the Wild-Type Form in Free-Living and Static Conditions but NotApplied and environmental microbiology · 2019Article
- Defining the Genetic Basis of Plant⁻Endophytic Bacteria Interactions.International journal of molecular sciences · 2019Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
The Rhizobium leguminosarum biovar viciae genome contains several genes predicted to determine surface polysaccharides. Mutants predicted to affect the initial steps of polysaccharide synthesis were identified and characterized. In addition to the known cellulose (cel) and acidic exopolysaccharide (EPS) (pss) genes, we mutated three other loci; one of these loci (gmsA) determines glucomannan synthesis and one (gelA) determines a gel-forming polysaccharide, but the role of the other locus (an exoY-like gene) was not identified. Mutants were tested for attachment and biofilm formation in vitro and on root hairs; the mutant lacking the EPS was defective for both of these characteristics, but mutation of gelA or the exoY-like gene had no effect on either type of attachment. The cellulose (celA) mutant attached and formed normal biofilms in vitro, but it did not form a biofilm on root hairs, although attachment did occur. The cellulose-dependent biofilm on root hairs appears not to be critical for nodulation, because the celA mutant competed with the wild-type for nodule infection. The glucomannan (gmsA) mutant attached and formed normal biofilms in vitro, but it was defective for attachment and biofilm formation on root hairs. Although this mutant formed nodules on peas, it was very strongly outcompeted by the wild type in mixed inoculations, showing that glucomannan is critical for competitive nodulation. The polysaccharide synthesis genes around gmsA are highly conserved among other rhizobia and agrobacteria but are absent from closely related bacteria (such as Brucella spp.) that are not normally plant associated, suggesting that these genes may play a wide role in bacterium-plant interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.