ArticleRNA (New York, N.Y.)2008
A conserved element that stabilizes the group II intron active site.
Article in RNA (New York, N.Y.), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Article
- The ChloroplastCells · 2021Review
- Article
- Article
- Group II introns: mobile ribozymes that invade DNA.Cold Spring Harbor perspectives in biology · 2011Review
- Exploration of the central dogma at the interface of chemistry and biology: 2010 Yale Chemical Biology Symposium.The Yale journal of biology and medicine · 2010Article
- The tertiary structure of group II introns: implications for biological function and evolution.Critical reviews in biochemistry and molecular biology · 2010Review
- Tertiary architecture of the Oceanobacillus iheyensis group II intron.RNA (New York, N.Y.) · 2010Article
- Article
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Authors and funding
2 authors.
Funding
Abstract
The internal loop at the base of domain 3 (D3) is one of the most conserved and catalytically important elements of a group II intron. However, the location and molecular nature of its tertiary interaction partners has remained unknown. By employing a combination of site-directed photo-cross-linking and nucleotide analog interference suppression (NAIS), we show that the domain 3 internal loop (D3IL) interacts with the epsilon-epsilon' duplex, which is an active-site element located near the 5'-splice site in D1. Our data also suggest that the D3IL may interact with the bulge of D5, which is a critical active site component. The results of this and other recent studies indicate that the D3IL participates in a complex network of tertiary interactions involving epsilon-epsilon', the bulge of D5 and J23, and that it helps to optimize active site architecture by supporting interactions among these catalytic motifs. Our results are consistent with the role of D3 as a catalytic effector that enhances intron reactivity through active site stabilization.
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Registered trials
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