ArticleBiophysical journal2008
Cooperativity and specificity in enzyme kinetics: a single-molecule time-based perspective.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Trade-offs and thermodynamics of energy-relay proofreading.Journal of the Royal Society, Interface · 2024Article
- A suicidal and extensively disordered luciferase with a bright luminescence.Protein science : a publication of the Protein Society · 2024Article
- Identifying the active sites in unequal iron-nitrogen single-atom catalysts.Nature communications · 2023Article
- Allosteric autoactivation of SOS and its kinetic mechanism.Small GTPases · 2021Article
- Dynamics and Location of the Allosteric Midazolam Site in Cytochrome P4503A4 in Lipid Nanodiscs.Biochemistry · 2020Article
- Quantifying the flux as the driving force for nonequilibrium dynamics and thermodynamics in non-Michaelis-Menten enzyme kinetics.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Extensions to Michaelis-Menten Kinetics for Single Parameters.Scientific reports · 2018Article
- Structural conditions on complex networks for the Michaelis-Menten input-output response.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- The uncoupled ATPase activity of the ABC transporter BtuC2D2 leads to a hysteretic conformational change, conformational memory, and improved activity.Scientific reports · 2016Article
- Statistics and Related Topics in Single-Molecule Biophysics.Annual review of statistics and its application · 2014Article
- Allosteric activation of the phosphoinositide phosphatase Sac1 by anionic phospholipids.Biochemistry · 2012Article
- Stochastic ensembles, conformationally adaptive teamwork, and enzymatic detoxification.Biochemistry · 2011Article
- Michaelis-Menten relations for complex enzymatic networks.The Journal of chemical physics · 2011Article
- Metallic-Nanostructure-Enhanced Fluorescence of Single Flavin Cofactor and Single Flavoenzyme Molecules.The journal of physical chemistry. C, Nanomaterials and interfaces · 2011Article
- The chemical master equation approach to nonequilibrium steady-state of open biochemical systems: linear single-molecule enzyme kinetics and nonlinear biochemical reaction networks.International journal of molecular sciences · 2010Review
- Rate theories for biologists.Quarterly reviews of biophysics · 2010Review
- Application of the principles of systems biology and Wiener's cybernetics for analysis of regulation of energy fluxes in muscle cells in vivo.International journal of molecular sciences · 2010Review
- Ligand depletion in vivo modulates the dynamic range and cooperativity of signal transduction.PloS one · 2010Article
- Assembly of multiprotein complexes that control genome function.The Journal of cell biology · 2009Review
Corrections and comments
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Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An alternative theoretical approach to enzyme kinetics that is particularly applicable to single-molecule enzymology is presented. The theory, originated by Van Slyke and Cullen in 1914, develops enzyme kinetics from a "time perspective" rather than the traditional "rate perspective" and emphasizes the nonequilibrium steady-state nature of enzymatic reactions and the significance of small copy numbers of enzyme molecules in living cells. Sigmoidal cooperative substrate binding to slowly fluctuating, monomeric enzymes is shown to arise from association pathways with very small probability but extremely long passage time, which would be disregarded in the traditional rate perspective: A single enzyme stochastically takes alternative pathways in serial order rather than different pathways in parallel. The theory unifies dynamic cooperativity and Hopfield-Ninio's kinetic proofreading mechanism for specificity amplification.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.