ArticleJournal of molecular biology2008
Structural basis for chitotetraose coordination by CGL3, a novel galectin-related protein from Coprinopsis cinerea.
Article in Journal of molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 61 citations in OpenAlex.
- From sugar binders to diabetes fighters: the lectin saga of antihyperglycemic activity through systematic review and meta-analysis.Frontiers in pharmacology · 2024Pooled it
- The Entomotoxic Fungal LectinJournal of agricultural and food chemistry · 2026Article
- Exposure to specific fungal lectins during adhesion impairs biofilm formation of Listeria on polystyrene.Microbial biotechnology · 2024Article
- Modulation ofFrontiers in cellular and infection microbiology · 2024Article
- Lessons on fruiting body morphogenesis from genomes and transcriptomes ofStudies in mycology · 2023Article
- Article
- Structure-function relationship of a novel fucoside-binding fruiting body lectin from Coprinopsis cinerea exhibiting nematotoxic activity.Glycobiology · 2022Article
- Highly modified and immunoactive N-glycans of the canine heartworm.Nature communications · 2019Article
- Article
- Galectin-related protein: An integral member of the network of chicken galectins 1. From strong sequence conservation of the gene confined to vertebrates to biochemical characteristics of the chicken protein and its crystal structure.Biochimica et biophysica acta · 2016Article
- Identification of a Novel Nematotoxic Protein by Challenging the Model Mushroom Coprinopsis cinerea with a Fungivorous Nematode.G3 (Bethesda, Md.) · 2015Article
- Inhibition of Haemonchus contortus larval development by fungal lectins.Parasites & vectors · 2015Article
- Hitting the sweet spot-glycans as targets of fungal defense effector proteins.Molecules (Basel, Switzerland) · 2015Review
- Mushroom lectins: specificity, structure and bioactivity relevant to human disease.International journal of molecular sciences · 2015Review
- Lectins from edible mushrooms.Molecules (Basel, Switzerland) · 2014Review
- Isolation and characterization of a novel lectin from the edible mushroom Stropharia rugosoannulata.Molecules (Basel, Switzerland) · 2014Article
- The galectin CvGal1 from the eastern oyster (Crassostrea virginica) binds to blood group A oligosaccharides on the hemocyte surface.The Journal of biological chemistry · 2013Article
- Array-assisted characterization of a fucosyltransferase required for the biosynthesis of complex core modifications of nematode N-glycans.The Journal of biological chemistry · 2013Article
- 5'-Serial Analysis of Gene Expression studies reveal a transcriptomic switch during fruiting body development in Coprinopsis cinerea.BMC genomics · 2013Article
- LEC-2, a highly variable lectin in the lichenSymbiosis (Philadelphia, Pa.) · 2012Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Recent advances in genome sequencing efforts have revealed an abundance of novel putative lectins. Among these, many galectin-related proteins, characterized by many conserved residues but intriguingly lacking critical amino acids, have been found in all corners of the eukaryotic superkingdom. Here we present a structural and biochemical analysis of one representative, the galectin-related lectin CGL3 found in the inky cap mushroom Coprinopsis cinerea. This protein contains all but one conserved residues known to be involved in beta-galactoside binding in galectins. A Trp residue strictly conserved among galectins is changed to an Arg in CGL3 (R81). Accordingly, the galectin-related protein is not able to bind lactose. Screening of a glycan array revealed that CGL3 displays preference for oligomers of beta1-4-linked N-acetyl-glucosamines (chitooligosaccharides) and GalNAc beta 1-4GlcNAc (LacdiNAc). Carbohydrate-binding affinity of this novel lectin was quantified using isothermal titration calorimetry, and its mode of chitooligosaccharide coordination not involving any aromatic amino acid residues was studied by X-ray crystallography. Structural information was used to alter the carbohydrate-binding specificity and substrate affinity of CGL3. The importance of residue R81 in determining the carbohydrate-binding specificity was demonstrated by replacing this Arg with a Trp residue (R81W). This single-amino-acid change led to a lectin that failed to bind chitooligosaccharides but gained lactose binding. Our results demonstrate that, similar to the legume lectin fold, the galectin fold represents a conserved structural framework upon which dramatically altered specificities can be grafted by few alterations in the binding site and that, in consequence, many metazoan galectin-related proteins may represent lectins with novel carbohydrate-binding specificities.
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