ArticleBiochimica et biophysica acta2008
A tightly regulated Pol III promoter for synthesis of miRNA genes in tandem.
Article in Biochimica et biophysica acta, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- Role of ncRNAs in the Development of Chronic Pain.Non-coding RNA · 2025Review
- High-accuracy crRNA array assembly strategy for multiplex CRISPR.Molecular therapy. Nucleic acids · 2025Article
- MicroRNAs Regulating Autophagy in Neurodegeneration.Advances in experimental medicine and biology · 2021Article
- Inducible Genetic Code Expansion in Eukaryotes.Chembiochem : a European journal of chemical biology · 2020Article
- miR-3174 Contributes to Apoptosis and Autophagic Cell Death Defects in Gastric Cancer Cells by Targeting ARHGAP10.Molecular therapy. Nucleic acids · 2017Article
- miRNA engineering of CHO cells facilitates production of difficult-to-express proteins and increases success in cell line development.Biotechnology and bioengineering · 2017Article
- Human RNAi pathway: crosstalk with organelles and cells.Functional & integrative genomics · 2014Review
- Functional silencing of guanylyl cyclase/natriuretic peptide receptor-A by microRNA interference: analysis of receptor endocytosis.International journal of biochemistry and molecular biology · 2013Article
- Optimization and comparison of knockdown efficacy between polymerase II expressed shRNA and artificial miRNA targeting luciferase and Apolipoprotein B100.BMC biotechnology · 2012Article
- Transcriptional regulation of mammalian miRNA genes.Genomics · 2011Review
- A direct comparison of strategies for combinatorial RNA interference.BMC molecular biology · 2010Article
- Deriving four functional anti-HIV siRNAs from a single Pol III-generated transcript comprising two adjacent long hairpin RNA precursors.Nucleic acids research · 2010Article
- Construction of an artificial MicroRNA expression vector for simultaneous inhibition of multiple genes in mammalian cells.International journal of molecular sciences · 2009Article
- Transcriptional control of microRNA expression in C. elegans: promoting better understanding.RNA biologyReview
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
A compelling tool for functional genetics is to silence the expression of multiple related genes concomitantly and reversibly. Such a tool will accelerate the understanding on gene interaction in signaling pathway and the development of comprehensive animal models for human diseases. Multiple gene silencing may be achieved by concurrent expression of multiple miRNA from a Pol II promoter. By comparison, Pol III promoters possess greater capacity to synthesize RNA of high yield and are consisted of compact elements and simple terminators to be convenient for handling. The miRNA-induced gene silencing is a dose-dependent event, and thus, Pol III promoter as a miRNA driver increases the chance to induce phenotypes subsequent to the gene silencing. As a Pol III promoter, endogenous U6 promoter synthesizes small nuclear RNA of high yield and is commonly adapted for miRNA synthesis. Whether U6 promoter is effective to synthesize multiple miRNA in tandem remains to be determined. This study exploited a possibility to express multiple miRNA genes from U6 promoter and also tested the inducibility of varying types of Tet-regulatable U6 promoters. With miR-30a backbone, two miRNA genes were functionally and efficiently expressed from a U6 promoter. The transcriptional activity of Tet-regulatable U6 promoter was tightly regulated by Tetracycline system after sufficient repeats of Tetracycline Operator sequence were introduced within the promoter regions and also between U6 promoter and miRNA gene. This newly developed U6 miRNA system would make multi-gene silencing efficient and reversible.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.