ArticleClinical orthopaedics and related research2008
Molecular identification of bacteria from aseptically loose implants.
Article in Clinical orthopaedics and related research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 2 of them syntheses that pooled it.
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Who cites it
38 citing papers in PubMed, 2 syntheses or guidelines pooled it, 87 citations in OpenAlex.
- Meta-analysis of sonication prosthetic fluid PCR for diagnosing periprosthetic joint infection.PloS one · 2018Pooled it
- PCR-based diagnosis of prosthetic joint infection.Journal of clinical microbiology · 2013Pooled it
- Current Status and Perspectives of Diagnosis and Treatment of Periprosthetic Joint Infection.Infection and drug resistance · 2024Review
- Decreased serum superoxide dismutase concentration has a high value for the diagnosis of periprosthetic joint infection-a single-center, retrospective study.BMC musculoskeletal disorders · 2022Article
- Sonication of explants enhances the diagnostic accuracy of synovial fluid and tissue cultures and can help determine the appropriate antibiotic therapy for prosthetic joint infections.International orthopaedics · 2022Article
- Determining the Clinical Utility of 16S rRNA Sequencing in the Management of Culture-Negative Pediatric Infections.Antibiotics (Basel, Switzerland) · 2022Article
- Suppression of NF-κB-induced chronic inflammation mitigates inflammatory osteolysis in the murine continuous polyethylene particle infusion model.Journal of biomedical materials research. Part A · 2021Article
- Sonication Leads to Clinically Relevant Changes in Treatment of Periprosthetic Hip or Knee Joint Infection.Journal of bone and joint infection · 2020Article
- Positive Microbiological Findings at the Site of Presumed Aseptic Revision Arthroplasty Surgery of the Hip and Knee Joint: Is a Surgical Revision Always Necessary?BioMed research international · 2020Article
- Precision Medicine in the Diagnosis and Management of Orthopedic Biofilm Infections.Frontiers in medicine · 2020Article
- Lack of Additional Diagnostic Yield of 16s rRNA Gene PCR for Prosthetic Joint Infections.The journal of applied laboratory medicine · 2019Article
- Microbial Identification Using DNA Target Amplification and Sequencing: Clinical Utility and Impact on Patient Management.Open forum infectious diseases · 2018Review
- Identification of Prosthetic Joint Infection Pathogens Using a Shotgun Metagenomics Approach.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2018Article
- NFκB sensing IL-4 secreting mesenchymal stem cells mitigate the proinflammatory response of macrophages exposed to polyethylene wear particles.Journal of biomedical materials research. Part A · 2018Article
- Emperor's new clothes: Is particle disease really infected particle disease?Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2016Review
- Multi-disciplinary antimicrobial strategies for improving orthopaedic implants to prevent prosthetic joint infections in hip and knee.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2016Review
- PCR diagnostic system in the treatment of prosthetic joint infections.Folia microbiologica · 2015Article
- Enrichment of bacteria samples by centrifugation improves the diagnosis of orthopaedics-related infections via real-time PCR amplification of the bacterial methicillin-resistance gene.BMC research notes · 2015Article
- Molecular diagnostics.The Journal of the American Academy of Orthopaedic Surgeons · 2015Article
- Is synovial C-reactive protein a useful marker for periprosthetic joint infection?Clinical orthopaedics and related research · 2014Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelledPolymerase chain reaction (PCR) assays have been used to detect bacteria adherent to failed orthopaedic implants, but some PCR assays have had problems with probable false-positive results. We used a combination of a Staphylococcus species-specific PCR and a universal PCR followed by DNA sequencing to identify bacteria on implants retrieved from 52 patients (92 implants) at revision arthroplasty. We addressed two questions in this study: (1) Is this method able to show the existence of bacterial DNA on presumed aseptic loosed implants?; and (2) What proportion of presumed aseptic or culture-negative implants was positive for bacterial DNA by PCR? Fourteen implants (15%) were believed infected, whereas 74 implants (85%) were believed aseptic. Each implant was sonicated and the resulting solution was submitted for dual real-time PCR assay and culture. All implants believed aseptically loose were culture-negative, but nine of the 74 (12%) had bacterial DNA by PCR; two (2.7%) were PCR-positive and also showed histologic findings suggestive of infection. Uniquely developed PCR and bacterial sequencing assays showed bacterial DNA on 12% of implants removed for presumed aseptic loosening. Additional studies are needed to determine the clinical importance of bacterial DNA detected by PCR but not by conventional culture. LEVEL OF EVIDENCE: Level III, diagnostic study.
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