ArticleTransgenic research2008
Excision of a selective marker in transgenic rice using a novel Cre/loxP system controlled by a floral specific promoter.
Article in Transgenic research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Application of a multiplex CRISPR/Cas9 strategy for elimination of selection markers from transgenic plants.Frontiers in genome editing · 2025Article
- Engineered Expression of Vip3A in Green Tissues as a Feasible Approach for the Control of Insect Pests in Maize.Insects · 2023Article
- Selectable Markers to Marker-Free Selection in Rice.Molecular biotechnology · 2022Review
- An Efficient Marker Gene Excision Strategy Based on CRISPR/Cas9-Mediated Homology-Directed Repair in Rice.International journal of molecular sciences · 2022Article
- A Prospective Review on Selectable Marker-Free Genome Engineered Rice: Past, Present and Future Scientific Realm.Frontiers in genetics · 2022Review
- Gene activation via Cre/lox-mediated excision in cowpea (Vigna unguiculata).Plant cell reports · 2022Article
- Generation of Marker-Free Transgenic Rice Resistant to Rice Blast Disease UsingFrontiers in plant science · 2021Article
- Selectable Marker Gene Removal and Expression of Transgene by Inducible Promoter Containing FFDDIranian journal of biotechnology · 2015Article
- The pollen- and embryo-specific Arabidopsis DLL promoter bears good potential for application in marker-free Cre/loxP self-excision strategy.Plant cell reports · 2015Article
- Split-Cre complementation restores combination activity on transgene excision in hair roots of transgenic tobacco.PloS one · 2014Article
- Ectopic expression of OsMADS45 activates the upstream genes Hd3a and RFT1 at an early development stage causing early flowering in rice.Botanical studies · 2013Article
- Excision of a selectable marker gene in transgenic banana using a Cre/lox system controlled by an embryo specific promoter.Plant molecular biology · 2013Article
- Utilization of PVX-Cre expression vector in potato.Transgenic research · 2012Article
- Recent advances in development of marker-free transgenic plants: regulation and biosafety concern.Journal of biosciences · 2012Review
- Development of a simple and efficient system for excising selectable markers in Arabidopsis using a minimal promoter::Cre fusion construct.Molecules and cells · 2012Article
- High-resolution genetic mapping and candidate gene identification of the SLP1 locus that controls glume development in rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2011Article
- Cre-mediated seed-specific transgene excision in tobacco.Plant molecular biology · 2010Article
- Selectable antibiotic resistance marker gene-free transgenic rice harbouring the garlic leaf lectin gene exhibits resistance to sap-sucking planthoppers.Plant cell reports · 2010Article
- A novel mannose-based selection system for plant transformation using celery mannose-6-phosphate reductase gene.Plant cell reports · 2010Article
- Generation of marker-free Bt transgenic indica rice and evaluation of its yellow stem borer resistance.Journal of applied genetics · 2010Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Based on the Cre/loxP system, we have developed a novel marker-free system mediating a direct auto-excision of loxP-flanked marker genes from T(1 )transgenic rice without any treatment or further offspring crossing. To achieve this, the floral-specific promoter OsMADS45 was isolated from rice and the expression patterns of OsMADS45 promoter was characterised by using the pOs45:GUS transgenic plants. Furthermore, the binary vector with Cre recombinase under the control of OsMADS45 promoter was constructed and introduced into rice by Agrobacterium-mediated transformation and transgenic rice plants were generated. Southern blot analysis showed that auto-excision of the selective markers occurred in some T(1) progeny of the transgenic plants, suggesting that a high auto-excision frequency can be achieved with our Cre/loxP system. This auto-excision strategy provides an efficient way of removing the selectable marker gene from transgenic rice.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.