ArticlePancreas2008
Small-interference RNA gene knockdown of pancreatitis-associated proteins in rat acute pancreatitis.
Article in Pancreas, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Who cites it
8 citing papers in PubMed, 15 citations in OpenAlex.
- Role of regenerating islet-derived proteins in inflammatory bowel disease.World journal of gastroenterology · 2020Review
- Mechanisms of interleukin-22's beneficial effects in acute pancreatitis.World journal of gastrointestinal pathophysiology · 2016Review
- Aryl hydrocarbon receptor regulates pancreatic IL-22 production and protects mice from acute pancreatitis.Gastroenterology · 2012Article
- Acute pancreatitis in aging animals: loss of pancreatitis-associated protein protection?World journal of gastroenterology · 2012Article
- Comparison of His and GST tagged versions of recombinant pancreatitis associated protein 2 in modulation of inflammatory responses.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2010Article
- Differential expression of pancreatitis-associated protein and thrombospondins in arterial versus venous tissues.Journal of vascular research · 2009Article
- Small-interference RNA gene knockdown of pancreatitis-associated proteins in rat acute pancreatitis.Pancreas · 2008Article
- Dexamethasone mediates protection against acute pancreatitis via upregulation of pancreatitis-associated proteins.World journal of gastroenterology · 2006Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
objectivesPancreatitis-associated proteins (PAPs) are induced in acute pancreatitis and antisense-mediated gene knockdown of PAP decreased PAP gene expression and worsened pancreatitis. Here, we investigated the effect of a more stable inhibition of PAP using small-interference RNA gene knockdown in vitro and in an in vivo model of experimental pancreatitis.
methodsPancreatitis-associated protein-specific siRNA was administered to AR42J cell cultures or rats induced with pancreatitis. Controls included administration of scrambled siRNA or vehicle alone. After 24 hours, cells and pancreata were harvested and assessed for PAP (PAP 1, PAP 2, PAP 3) gene expression and pancreatitis severity.
resultsIn vitro, PAP protein, and mRNA levels were reduced (PAP 1, 76%; PAP 2, 8%; PAP 3, 24%) in cells treated with PAP siRNA. In vivo, PAP 1, and PAP 3 expressions were reduced (PAP 1, 36%; PAP 3, 66%) in siRNA-treated rats; there was no difference in PAP 2 isoform mRNA expression and serum protein levels. Serum amylase and lipase levels decreased (> or =50%) after administration of siRNA; interleukin (IL) 1beta, IL-4, and IL-6 increased, whereas C-reactive protein and tumor necrosis factor-alpha decreased when compared with vehicle control. Administration of PAP siRNA correlated with worsening histopathology.
conclusionssiRNA-mediated gene knockdown of PAP worsens pancreatitis. Differences in gene knockdown technology may provide different approaches to study gene function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.