ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2008
Cholangiocyte expression of alpha2beta1-integrin confers susceptibility to rotavirus-induced experimental biliary atresia.
Article in American journal of physiology. Gastrointestinal and liver physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 46 citations in OpenAlex.
- IL‑17AInternational journal of molecular medicine · 2026Article
- Specific binding sites on Rhesus rotavirus capsid protein dictate the method of endocytosis inducing the murine model of biliary atresia.American journal of physiology. Gastrointestinal and liver physiology · 2024Article
- Deletion of Interferon Lambda Receptor Elucidates Susceptibility to the Murine Model of Biliary Atresia.Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research · 2023Article
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- Evidence for Viral Induction of Biliary Atresia: A Review.Journal of clinical and translational hepatology · 2018Review
- Role of viruses in biliary atresia: news from mice and men.Innovative surgical sciences · 2018Review
- Gene and Protein Expression Profiles in a Mouse Model of Collagen-Induced Arthritis.International journal of medical sciences · 2018Article
- A Point Mutation in the Rhesus Rotavirus VP4 Protein Generated through a Rotavirus Reverse Genetics System Attenuates Biliary Atresia in the Murine Model.Journal of virology · 2017Article
- The SRL peptide of rhesus rotavirus VP4 protein governs cholangiocyte infection and the murine model of biliary atresia.Hepatology (Baltimore, Md.) · 2017Article
- Biliary atresia: Where do we stand now?World journal of hepatology · 2016Review
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- Rhesus rotavirus VP4 sequence-specific activation of mononuclear cells is associated with cholangiopathy in murine biliary atresia.American journal of physiology. Gastrointestinal and liver physiology · 2015Article
- Pathogenesis of biliary atresia: defining biology to understand clinical phenotypes.Nature reviews. Gastroenterology & hepatology · 2015Review
- The Sea Lamprey as an Etiological Model for Biliary Atresia.BioMed research international · 2015Review
- Integrative genomics identifies candidate microRNAs for pathogenesis of experimental biliary atresia.BMC systems biology · 2013Article
- Aetiology of biliary atresia: what is actually known?Orphanet journal of rare diseases · 2013Review
- Rotavirus replication in the cholangiocyte mediates the temporal dependence of murine biliary atresia.PloS one · 2013Article
- Clues to the etiology of bile duct injury in biliary atresia.Seminars in liver disease · 2012Review
- Rotavirus infection of human cholangiocytes parallels the murine model of biliary atresia.The Journal of surgical research · 2012Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Inoculation of BALB/c mice with rhesus rotavirus (RRV) in the newborn period results in biliary epithelial cell (cholangiocyte) infection and the murine model of biliary atresia. Rotavirus infection of a cell requires attachment, which is governed in part by cell-surface expression of integrins such as alpha2beta1. We hypothesized that cholangiocytes were susceptible to RRV infection because they express alpha2beta1. RRV attachment and replication was measured in cell lines derived from cholangiocytes and hepatocytes. Flow cytometry was performed on these cell lines to determine whether alpha2beta1 was present. Cholangiocytes were blocked with natural ligands, a monoclonal antibody, or small interfering RNA against the alpha2-subunit and were infected with RRV. The extrahepatic biliary tract of newborn mice was screened for the expression of the alpha2beta1-integrin. Newborn mice were pretreated with a monoclonal antibody against the alpha2-subunit and were inoculated with RRV. RRV attached and replicated significantly better in cholangiocytes than in hepatocytes. Cholangiocytes, but not hepatocytes, expressed alpha2beta1 in vitro and in vivo. Blocking assays led to a significant reduction in attachment and yield of virus in RRV-infected cholangiocytes. Pretreatment of newborn pups with an anti-alpha2 monoclonal antibody reduced the ability of RRV to cause biliary atresia in mice. Cell-surface expression of the alpha2beta1-integrin plays a role in the mechanism that confers cholangiocyte susceptibility to RRV infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.