ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2008
Dynamics of somatostatin type 2A receptor cargoes in living hippocampal neurons.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- The beta1-adrenergic receptor in the heart.Cell death discovery · 2025Review
- A simple novel approach for detecting blood-brain barrier permeability using GPCR internalization.Neuropathology and applied neurobiology · 2021Article
- International Union of Basic and Clinical Pharmacology. CV. Somatostatin Receptors: Structure, Function, Ligands, and New Nomenclature.Pharmacological reviews · 2018Review
- Endocytosis of Activated Muscarinic m2 Receptor (m2R) in Live Mouse Hippocampal Neurons Occurs via a Clathrin-Dependent Pathway.Frontiers in cellular neuroscience · 2018Article
- A New Mechanism of Receptor Targeting by Interaction between Two Classes of Ligand-Gated Ion Channels.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Trans-Modulation of the Somatostatin Type 2A Receptor Trafficking by Insulin-Regulated Aminopeptidase Decreases Limbic Seizures.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Subcellular localization of coagulation factor II receptor-like 1 in neurons governs angiogenesis.Nature medicine · 2014Article
- Mobility of acetylcholine receptors in command Helix lucorum neurons in a cellular analog of habituation.Invertebrate neuroscience : IN · 2013Article
- Agonist-biased trafficking of somatostatin receptor 2A in enteric neurons.The Journal of biological chemistry · 2013Article
- Sstr2A: a relevant target for the delivery of genes into human glioblastoma cells using fiber-modified adenoviral vectors.Gene therapy · 2013Article
- A new vesicular scaffolding complex mediates the G-protein-coupled 5-HT1A receptor targeting to neuronal dendrites.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Trans-Golgi Network (TGN) as a regulatory node for β1-adrenergic receptor (β1AR) down-modulation and recycling.The Journal of biological chemistry · 2012Article
- The rates of protein synthesis and degradation account for the differential response of neurons to spaced and massed training protocols.PLoS computational biology · 2011Article
- The N terminus of the adhesion G protein-coupled receptor GPR56 controls receptor signaling activity.The Journal of biological chemistry · 2011Article
- Intracellular α(2C)-adrenoceptors: storage depot, stunted development or signaling domain?Biochimica et biophysica acta · 2011Review
- Down-modulation of the G-protein-coupled estrogen receptor, GPER, from the cell surface occurs via a trans-Golgi-proteasome pathway.The Journal of biological chemistry · 2011Article
- CC chemokine receptor 5 (CCR5) desensitization: cycling receptors accumulate in the trans-Golgi network.The Journal of biological chemistry · 2010Article
- GPR54 regulates ERK1/2 activity and hypothalamic gene expression in a Gα(q/11) and β-arrestin-dependent manner.PloS one · 2010Article
- Article
- Targeting of the 5-HT1A serotonin receptor to neuronal dendrites is mediated by Yif1B.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
Corrections and comments
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Authors and funding
11 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite the large number of G-protein-coupled receptor (GPCR) types expressed in the CNS, little is known about their dynamics in neuronal cells. Dynamic properties of the somatostatin type 2A receptor were therefore examined in resting conditions and after agonist activation in living hippocampal neurons. Using fluorescence recovery after photobleaching experiments, we found that, in absence of ligand, the sst(2A) receptor is mobile and laterally and rapidly diffuse in neuronal membranes. We then observed by live-cell imaging that, after agonist activation, membrane-associated receptors induce the recruitment of beta-arrestin 1-enhanced green fluorescent protein (EGFP) and beta-arrestin 2-EGFP to the plasma membrane. In addition, beta-arrestin 1-EGFP translocate to the nucleus, suggesting that this protein could serve as a nuclear messenger for the sst(2A) receptor in neurons. Receptors are then recruited to preexisting clathrin coated pits, form clusters that internalize, fuse, and move to a perinuclear compartment that we identified as the trans-Golgi network (TGN), and recycle. Receptor cargoes are transported through a microtubule-dependent process directly from early endosomes/recycling endosomes to the TGN, bypassing the late endosomal compartment. Together, these results provide a comprehensive description of GPCR trafficking in living neurons and provide compelling evidence that GPCR cargoes can recycle through the TGN after endocytosis, a phenomenon that has not been anticipated from studies of non-neuronal cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.