ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2008
Abundance and stability of complexes containing inactive G protein-coupled receptors and G proteins.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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Who cites it
33 citing papers in PubMed, 57 citations in OpenAlex.
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- Chronic Administrations of Guanfacine on Mesocortical Catecholaminergic and Thalamocortical Glutamatergic Transmissions.International journal of molecular sciences · 2021Article
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- Employing Genetically Encoded, Biophysical Sensors to Understand WNT/Frizzled Interaction and Receptor Complex Activation.Handbook of experimental pharmacology · 2021Article
- The Influence of Psychological Stress on the Initiation and Progression of Diabetes and Cancer.International journal of endocrinology and metabolism · 2019Review
- Noradrenergic targets for the treatment of alcohol use disorder.Psychopharmacology · 2018Review
- Overview of the Microenvironment of Vasculature in Vascular Tone Regulation.International journal of molecular sciences · 2018Review
- Serotonin Receptor Binding Characteristics of Geissoschizine Methyl Ether, an Indole Alkaloid in Uncaria Hook.Current medicinal chemistry · 2018Review
- The Cholinergic and Adrenergic Autocrine Signaling Pathway Mediates Immunomodulation in OysterFrontiers in immunology · 2018Article
- Agonist-induced dimer dissociation as a macromolecular step in G protein-coupled receptor signaling.Nature communications · 2017Article
- The G protein GThe Journal of biological chemistry · 2017Article
- Involvement of histaminergic and noradrenergic receptors in the oxytocin-induced food intake in neonatal meat-type chicks.Veterinary research communications · 2017Article
- WNT Stimulation Dissociates a Frizzled 4 Inactive-State Complex with Gα12/13.Molecular pharmacology · 2016Article
- Assessing Gonadotropin Receptor Function by Resonance Energy Transfer-Based Assays.Frontiers in endocrinology · 2015Article
- Desensitization of functional µ-opioid receptors increases agonist off-rate.Molecular pharmacology · 2014Article
- Structural Aspects of GPCR-G Protein Coupling.Toxicological research · 2013Article
- Interaction of Protease-Activated Receptor 2 with G Proteins and β-Arrestin 1 Studied by Bioluminescence Resonance Energy Transfer.Frontiers in endocrinology · 2013Article
- Assembly of membrane-bound protein complexes: detection and analysis by single molecule diffusion.Biochemistry · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
G protein-coupled receptors (GPCRs) interact directly with heterotrimeric G proteins to transduce physiological signals. Early studies of this interaction concluded that GPCRs (R) and G proteins (G) collide with each other randomly after receptor activation and that R-G complexes are transient. More recent studies have suggested that inactive R and G are preassembled (precoupled) as stable R-G complexes. Here we examine the stability of complexes formed between cyan fluorescent protein-labeled alpha(2A)-adrenoreceptors (C-alpha2ARs) and G proteins in cells using fluorescence recovery after photobleaching (FRAP). Labeled G proteins diffused in the plasma membrane with equal mobility in the absence and presence of immobile C-alpha2ARs. Immobile C-alpha2ARs activated labeled G proteins, demonstrating functional coupling without stable physical association. In contrast, a stable R-G interaction was detected when G proteins were deprived of nucleotides and C-alpha2ARs were active, as predicted by the ternary complex model. Overexpression of regulator of G protein signaling 4 (RGS4) accelerated the onset of effector activation but did not detectably alter the interaction between C-alpha2ARs and G proteins. We conclude that at most a small fraction of C-alpha2ARs and G proteins exist as R-G complexes at any moment.
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Registered trials
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