ArticleGene therapy2008
Lentiviral transduction of the murine lung provides efficient pseudotype and developmental stage-dependent cell-specific transgene expression.
Article in Gene therapy, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 54 citations in OpenAlex.
- Gene modification therapies for hereditary diseases in the fetus.Prenatal diagnosis · 2023Review
- Multiphoton intravital microscopy of rodents.Nature reviews. Methods primers · 2022Article
- Gene Therapy: Will the Promise of Optimizing Lung Allografts Become Reality?Frontiers in immunology · 2022Review
- ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactions.PLoS pathogens · 2021Article
- Use of Precision-Cut Lung Slices as anMolecular therapy. Methods & clinical development · 2018Article
- In vivo bioimaging with tissue-specific transcription factor activated luciferase reporters.Scientific reports · 2015Article
- In utero lung gene transfer using adeno-associated viral and lentiviral vectors in mice.Human gene therapy methods · 2014Article
- Restoring ciliary function to differentiated primary ciliary dyskinesia cells with a lentiviral vector.Gene therapy · 2014Article
- Harnessing alveolar macrophages for sustained mucosal T-cell recall confers long-term protection to mice against lethal influenza challenge without clinical disease.Mucosal immunology · 2014Article
- Lentiviral vector-based insertional mutagenesis identifies genes associated with liver cancer.Nature methods · 2013Article
- Intrapulmonary Versus Nasal Transduction of Murine Airways With GP64-pseudotyped Viral Vectors.Molecular therapy. Nucleic acids · 2013Article
- High efficiency gene transfer to airways of mice using influenza hemagglutinin pseudotyped lentiviral vectors.The journal of gene medicine · 2013Article
- Integrase-deficient lentiviral vectors mediate efficient gene transfer to human vascular smooth muscle cells with minimal genotoxic risk.Human gene therapy · 2012Article
- Jaagsiekte sheep retrovirus pseudotyped lentiviral vector-mediated gene transfer to fetal ovine lung.Gene therapy · 2012Article
- Genetic therapies for cystic fibrosis lung disease.Human molecular genetics · 2011Review
- Genetic modification of airway progenitors after lentiviral gene delivery to the amniotic fluid of murine fetuses.American journal of respiratory cell and molecular biology · 2011Article
- Gene delivery of a mutant TGFβ3 reduces markers of scar tissue formation after cutaneous wounding.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
- Article
- Respiratory syncytial virus engineered to express the cystic fibrosis transmembrane conductance regulator corrects the bioelectric phenotype of human cystic fibrosis airway epithelium in vitro.Journal of virology · 2010Article
- Lentiviral vectors and cystic fibrosis gene therapy.Viruses · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Gene transfer for cystic fibrosis (CF) airway disease has been hampered by the lung's innate refractivity to pathogen infection. We hypothesized that early intervention with an integrating gene transfer vector capable of transducing the lung via the lumen may be a successful therapeutic approach. An HIV-based lentiviral vector pseudotyped with the baculovirus gp64 envelope was applied to the fetal, neonatal or adult airways. Fetal intra-amniotic administration resulted in transduction of approximately 14% of airway epithelial cells, including both ciliated and non-ciliated epithelia of the upper, mid and lower airways; there was negligible alveolar or nasal transduction. Following neonatal intra-nasal administration we observed significant transduction of the airway epithelium (approximately 11%), although mainly in the distal lung, and substantial alveolar transduction. This expression was still detectable at 1 year after application. In the adult, the majority of transduction was restricted to the alveoli. In contrast, vesicular stomatitis virus glycoprotein pseudotyped virus transduced only alveoli after adult and neonatal application and no transduction was observed after fetal administration. Repeat administration did not increase transduction levels of the conducting airway epithelia. These data demonstrate that application at early developmental stages in conjunction with an appropriately pseudotyped virus provides efficient, high-level transgene expression in the murine lung. This may provide a modality for treatment for lung disease in CF.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.