ArticleJournal of biomedical materials research. Part B, Applied biomaterials2008
Osteogenic differentiation of mesenchymal stem cells in defined protein beads.
Article in Journal of biomedical materials research. Part B, Applied biomaterials, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 48 citations in OpenAlex.
- Mechanoactivation of Single Stem Cells in Microgels Using a 3D-Printed Stimulation Device.Small methods · 2024Article
- Prokaryotic Collagen-Like Proteins as Novel Biomaterials.Frontiers in bioengineering and biotechnology · 2022Review
- Modular microcarrier technologies for cell-based bone regeneration.Journal of materials chemistry. B · 2020Review
- Cell-Laden Agarose-Collagen Composite Hydrogels for Mechanotransduction Studies.Frontiers in bioengineering and biotechnology · 2020Article
- Influence of collagen-based integrin αJournal of biomedical materials research. Part A · 2018Article
- Review
- Stress-stiffening-mediated stem-cell commitment switch in soft responsive hydrogels.Nature materials · 2016Article
- Collagen Type II enhances chondrogenic differentiation in agarose-based modular microtissues.Cytotherapy · 2016Article
- Hydrogels That Allow and Facilitate Bone Repair, Remodeling, and Regeneration.Journal of materials chemistry. B · 2015Article
- Strategies for directing the structure and function of three-dimensional collagen biomaterials across length scales.Acta biomaterialia · 2014Review
- Comparison of uncultured marrow mononuclear cells and culture-expanded mesenchymal stem cells in 3D collagen-chitosan microbeads for orthopedic tissue engineering.Tissue engineering. Part A · 2014Article
- Winner for outstanding research in the Ph.D. category for the 2013 Society for Biomaterials meeting and exposition, April 10-13, 2013, Boston, Massachusetts: Osteogenic differentiation of adipose-derived and marrow-derived mesenchymal stem cells in modular protein/ceramic microbeads.Journal of biomedical materials research. Part A · 2013Article
- Assembly of discrete collagen-chitosan microenvironments into multiphase tissue constructs.Advanced healthcare materials · 2013Article
- Delivery of mesenchymal stem cells in chitosan/collagen microbeads for orthopedic tissue repair.Cells, tissues, organs · 2013Article
- Noninvasive, quantitative, spatiotemporal characterization of mineralization in three-dimensional collagen hydrogels using high-resolution spectral ultrasound imaging.Tissue engineering. Part C, Methods · 2012Article
- Differentiation of human mesenchymal stem cell spheroids under microgravity conditions.Cell regeneration (London, England) · 2012Article
- Microscale mechanisms of agarose-induced disruption of collagen remodeling.Biomaterials · 2011Article
- Mechanical and structural contribution of non-fibrillar matrix in uniaxial tension: a collagen-agarose co-gel model.Annals of biomedical engineering · 2011Article
- Article
- Phase-separated chitosan-fibrin microbeads for cell delivery.Journal of microencapsulation · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
There is a need to develop improved methods for directing and maintaining the differentiation of human mesenchymal stem cells (hMSC) for regenerative medicine. Here, we present a method for embedding cells in defined protein microenvironments for the directed osteogenic differentiation of hMSC. Composite matrices of collagen I and agarose were produced by emulsification and simultaneous polymerization in the presence of hMSC to produce 30-150 mum diameter hydrogel "beads." The proliferation, morphology, osteogenic gene expression, and calcium deposition of hMSC in bead environments were compared to other two- and three-dimensional culture environments over 14-21 days in culture. Cells embedded within 40% collagen beads exhibited equivalent proliferation rates to those in gel disks, but showed upregulation of bone sialoprotein and increased calcium deposition over 2D controls. Osteocalcin gene expression was not changed in 3D beads and disks, while collagen type I gene expression was downregulated relative to cells in 2D culture. The hydrogel bead format allows controlled cell differentiation and is a cell delivery vehicle that may also enhance vascular invasion and host incorporation. Our results indicate that the application of such beads can be used to promote the osteogenic phenotype in hMSC, which is an important step toward using them in bone repair applications.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.