ArticlePloS one2008
SAM domain-based protein oligomerization observed by live-cell fluorescence fluctuation spectroscopy.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Characterization of anPathogens (Basel, Switzerland) · 2023Article
- The Adaptor Protein UvSte50 Governs Fungal Pathogenicity ofJournal of fungi (Basel, Switzerland) · 2022Article
- Optimal fluorescent protein tags for quantifying protein oligomerization in living cells.Scientific reports · 2018Article
- Loss of a highly conserved sterile alpha motif domain gene (Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Scanning number and brightness yields absolute protein concentrations in live cells: a crucial parameter controlling functional bio-molecular interaction networks.Biophysical reviews · 2018Review
- Deciphering CaMKII Multimerization Using Fluorescence Correlation Spectroscopy and Homo-FRET Analysis.Biophysical journal · 2017Article
- Oligomeric state regulated trafficking of human platelet-activating factor acetylhydrolase type-II.Biochimica et biophysica acta · 2015Article
- Measuring protein dynamics in live cells: protocols and practical considerations for fluorescence fluctuation microscopy.Journal of biomedical optics · 2014Article
- Review
- Article
- Determining absolute protein numbers by quantitative fluorescence microscopy.Methods in cell biology · 2014Article
- Quantitative measurement of brightness from living cells in the presence of photodepletion.PloS one · 2014Article
- Roles of EphA2 in Development and Disease.Genes · 2013Article
- Multiple motifs regulate apical sorting of p75 via a mechanism that involves dimerization and higher-order oligomerization.Molecular biology of the cell · 2013Article
- Factors affecting the quantification of biomolecular interactions by fluorescence cross-correlation spectroscopy.Biophysical journal · 2012Article
- Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK.Structure (London, England : 1993) · 2011Article
- Ste11p MEKK signals through HOG, mating, calcineurin and PKC pathways to regulate the FKS2 gene.BMC molecular biology · 2011Article
- Article
- Ste50 adaptor protein governs sexual differentiation of Cryptococcus neoformans via the pheromone-response MAPK signaling pathway.Fungal genetics and biology : FG & B · 2011Article
- Toward quantitative "in vivo biochemistry" with fluorescence fluctuation spectroscopy.Molecular biology of the cell · 2010Review
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5 authors.
Funding
Abstract
Sterile-alpha-motif (SAM) domains are common protein interaction motifs observed in organisms as diverse as yeast and human. They play a role in protein homo- and hetero-interactions in processes ranging from signal transduction to RNA binding. In addition, mutations in SAM domain and SAM-mediated oligomers have been linked to several diseases. To date, the observation of heterogeneous SAM-mediated oligomers in vivo has been elusive, which represents a common challenge in dissecting cellular biochemistry in live-cell systems. In this study, we report the oligomerization and binding stoichiometry of high-order, multi-component complexes of (SAM) domain proteins Ste11 and Ste50 in live yeast cells using fluorescence fluctuation methods. Fluorescence cross-correlation spectroscopy (FCCS) and 1-dimensional photon counting histogram (1dPCH) confirm the SAM-mediated interaction and oligomerization of Ste11 and Ste50. Two-dimensional PCH (2dPCH), with endogenously expressed proteins tagged with GFP or mCherry, uniquely indicates that Ste11 and Ste50 form a heterogeneous complex in the yeast cytosol comprised of a dimer of Ste11 and a monomer of Ste50. In addition, Ste50 also exists as a high order oligomer that does not interact with Ste11, and the size of this oligomer decreases in response to signals that activate the MAP kinase cascade. Surprisingly, a SAM domain mutant of Ste50 disrupted not only the Ste50 oligomers but also Ste11 dimerization. These results establish an in vivo model of Ste50 and Ste11 homo- and hetero-oligomerization and highlight the usefulness of 2dPCH for quantitative dissection of complex molecular interactions in genetic model organisms such as yeast.
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