ArticleMolecular therapy : the journal of the American Society of Gene Therapy2008
Adult bone marrow-derived cells do not acquire functional attributes of cardiomyocytes when transplanted into peri-infarct myocardium.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 51 citations in OpenAlex.
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- The modest outcome of clinical trials with bone marrow cells for myocardial repair: is the autologous source of cells the prime culprit?Journal of thoracic disease · 2016Article
- Cardiac Repair and Regeneration: The Value of Cell Therapies.European cardiology · 2016Article
- c-kit(+) cells: the tell-tale heart of cardiac regeneration?Cellular and molecular life sciences : CMLS · 2015Review
- In vitro evaluation of endothelial progenitor cells from adipose tissue as potential angiogenic cell sources for bladder angiogenesis.PloS one · 2015Article
- Intramyocardial cell delivery: observations in murine hearts.Journal of visualized experiments : JoVE · 2014Article
- Micron-scale voltage and [Ca(2+)]i imaging in the intact heart.Frontiers in physiology · 2014Review
- Mouse adipose tissue stromal cells give rise to skeletal and cardiomyogenic cell sub-populations.Frontiers in cell and developmental biology · 2014Article
- Functional screening of intracardiac cell transplants using two-photon fluorescence microscopy.Pediatric cardiology · 2012Review
- The effect of erythropoietin on normal and neoplastic cells.Biologics : targets & therapy · 2012Article
- Challenges for heart disease stem cell therapy.Vascular health and risk management · 2012Review
- Capturing the stem cell paracrine effect using heparin-presenting nanofibres to treat cardiovascular diseases.Journal of tissue engineering and regenerative medicine · 2010Article
- CD117-positive cells of the heart: progenitor cells or mast cells?The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2010Article
- Delayed enrichment of mesenchymal cells promotes cardiac lineage and calcium transient development.Journal of molecular and cellular cardiology · 2010Article
- Interrogating functional integration between injected pluripotent stem cell-derived cells and surrogate cardiac tissue.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Assessment and optimization of cell engraftment after transplantation into the heart.Circulation research · 2010Review
- Bone marrow mononuclear stem cells: potential in the treatment of myocardial infarction.Stem cells and cloning : advances and applications · 2009Review
- MEK, p38, and PI-3K mediate cross talk between EGFR and TNFR in enhancing hepatocyte growth factor production from human mesenchymal stem cells.American journal of physiology. Cell physiology · 2009Article
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5 authors at 2 institutions in 1 country.
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Abstract
The cardiomyogenic potential of adult bone marrow (BM) cells after being directly transplanted into the ischemically injured heart remains a controversial issue. In this study, we investigated the ability of transplanted BM cells to develop intracellular calcium ([Ca(2+)](i)) transients in response to membrane depolarization in situ. Low-density mononuclear (LDM) BM cells, c-kit-enriched (c-kit(enr)) BM cells, and highly enriched lin(-) c-kit(+) BM cells were obtained from adult transgenic mice ubiquitously expressing enhanced green fluorescent protein (EGFP), and injected into peri-infarct myocardiums of nontransgenic mice. After 9-10 days the mice were killed, and the hearts were removed, perfused in Langendorff mode, loaded with the calcium-sensitive fluorophore rhod-2, and subjected to two-photon laser scanning fluorescence microscopy (TPLSM) to monitor action potential-induced [Ca(2+)](i) transients in EGFP-expressing donor-derived cells and non-expressing host cardiomyocytes. Whereas spontaneous and electrically evoked [Ca(2+)](i) transients were found to occur synchronously in host cardiomyocytes along the graft-host border and in areas remote from the infarct, they were absent in all of the >3,000 imaged BM-derived cells that were located in clusters throughout the infarct scar or peri-infarct zone. We conclude that engrafted BM-derived cells lack attributes of functioning cardiomyocytes, calling into question the concept that adult BM cells can give rise to substantive cardiomyocyte regeneration within the infarcted heart.
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