ArticleGenetics2008
The protease activity of yeast separase (esp1) is required for anaphase spindle elongation independently of its role in cleavage of cohesin.
Article in Genetics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- An evolutionarily conserved role for separase in the regulation of nuclear lamins.Cell death discovery · 2025Article
- Article
- APC/CCdc20-mediated degradation of Clb4 prompts astral microtubule stabilization at anaphase onset.The Journal of cell biology · 2023Article
- Mapping separase-mediated cleavage in situ.NAR genomics and bioinformatics · 2022Article
- Structure and Function of the Separase-Securin Complex.Sub-cellular biochemistry · 2021Review
- PP2AJournal of cell science · 2020Article
- Transcriptome analysis of the growth performance of hybrid mandarin fish after food conversion.PloS one · 2020Article
- Conservation of the separase regulatory domain.Biology direct · 2018Article
- Genetic Identification of Separase Regulators inG3 (Bethesda, Md.) · 2018Article
- A role for the budding yeast separase, Esp1, in Ty1 element retrotransposition.PLoS genetics · 2015Article
- Chromosome segregation in plant meiosis.Frontiers in plant science · 2014Review
- Article
- Post-replicative repair involves separase-dependent removal of the kleisin subunit of cohesin.Nature · 2013Article
- Unrestrained spindle elongation during recovery from spindle checkpoint activation in cdc15-2 cells results in mis-segregation of chromosomes.Molecular biology of the cell · 2010Article
- A role for separase in the regulation of RAB-11-positive vesicles at the cleavage furrow and midbody.Current biology : CB · 2010Article
- Arabidopsis separase functions beyond the removal of sister chromatid cohesion during meiosis.Plant physiology · 2009Article
- Smc5-Smc6-dependent removal of cohesin from mitotic chromosomes.Molecular and cellular biology · 2009Article
- Cdc14 inhibition by the spindle assembly checkpoint prevents unscheduled centrosome separation in budding yeast.Molecular biology of the cell · 2009Article
- Pds1p is required for meiotic recombination and prophase I progression in Saccharomyces cerevisiae.Genetics · 2009Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Separase is a caspase-family protease required for the metaphase-anaphase transition in eukaryotes. In budding yeast, the separase ortholog, Esp1, has been shown to cleave a subunit of cohesin, Mcd1 (Scc1), thereby releasing sister chromatids from cohesion and allowing anaphase. However, whether Esp1 has other substrates required for anaphase has been controversial. Whereas it has been reported that cleavage of Mcd1 is sufficient to trigger anaphase in the absence of Esp1 activation, another study using a temperature-sensitive esp1 mutant concluded that depletion of Mcd1 was not sufficient for anaphase in the absence of Esp1 function. Here we revisit the issue and demonstrate that neither depletion of Mcd1 nor ectopic cleavage of Mcd1 by Tev1 protease is sufficient to support anaphase in an esp1 temperature-sensitive mutant. Furthermore, we demonstrate that the catalytic activity of the Esp1 protease is required for this Mcd1-independent anaphase function. These data suggest that another protein, possibly a spindle-associated protein, is cleaved by Esp1 to allow anaphase. Such a function is consistent with the previous observation that Esp1 localizes to the mitotic spindle during anaphase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.