ArticleBMC plant biology2008
Characterisation of the tryptophan synthase alpha subunit in maize.
Article in BMC plant biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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26 citing papers in PubMed, 1 synthesis or guideline pooled it, 65 citations in OpenAlex.
- Tryptophan metabolism and plant health: mechanisms of action and application prospects.Frontiers in plant science · 2026Pooled it
- Evolutionary divergence and regulatory landscapes of benzoxazinoid metabolism in monocot crops.Plant cell reports · 2026Review
- A pseudoenzyme enables indole biosynthesis in eudicot plants.Nature chemical biology · 2026Article
- An auxin research odyssey: 1989-2023.The Plant cell · 2024Review
- Comparative Coexpression Analysis of Indole Synthase and Tryptophan Synthase A Reveals the Independent Production of Auxin via the Cytosolic Free Indole.Plants (Basel, Switzerland) · 2023Article
- MicroRNA172b-5p/trehalose-6-phosphate synthase module stimulates trehalose synthesis and microRNA172b-3p/AP2-like module accelerates flowering in barley upon drought stress.Frontiers in plant science · 2023Article
- Molecular cloning and functional characterization of BcTSA in the biosynthesis of indole alkaloids inFrontiers in plant science · 2023Article
- Application of data fusion modeling for the prediction of auxin response elements in Zea mays for food security purposes.Genomics & informatics · 2022Article
- Transcriptomic analysis of deceptively pollinated Arum maculatum (Araceae) reveals association between terpene synthase expression in floral trap chamber and species-specific pollinator attraction.G3 (Bethesda, Md.) · 2022Article
- Tryptophan Metabolites as Mediators of Microbiota-Gut-Brain Communication: Focus on Isatin.Frontiers in behavioral neuroscience · 2022Review
- Synthesis and regulation of auxin and abscisic acid in maize.Plant signaling & behavior · 2021Review
- Protocol: analytical methods for visualizing the indolic precursor network leading to auxin biosynthesis.Plant methods · 2021Article
- Quaternary Structure of the Tryptophan Synthase α-Subunit Homolog BX1 fromJournal of the American Society for Mass Spectrometry · 2020Article
- Generation of a Stand-Alone Tryptophan Synthase α-Subunit by Mimicking an Evolutionary Blueprint.Chembiochem : a European journal of chemical biology · 2019Article
- Contrasting insect attraction and herbivore-induced plant volatile production in maize.Planta · 2018Article
- Rapid defense responses in maize leaves induced by Spodoptera exigua caterpillar feeding.Journal of experimental botany · 2017Article
- Identification and VIGS-based characterization of Bx1 ortholog in rye (Secale cereale L.).PloS one · 2017Article
- Cloning and characterization of indole synthase (INS) and a putative tryptophan synthase α-subunit (TSA) genes from Polygonum tinctorium.Plant cell reports · 2016Article
- Joint-linkage mapping and GWAS reveal extensive genetic loci that regulate male inflorescence size in maize.Plant biotechnology journal · 2016Article
- Tryptophan-Independent Indole-3-Acetic Acid Synthesis: Critical Evaluation of the Evidence.Plant physiology · 2015Article
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Authors and funding
7 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundIn bacteria, such as Salmonella typhimurium, tryptophan is synthesized from indole-3-glycerole phosphate (IGP) by a tryptophan synthase alphabetabetaalpha heterotetramer. Plants have evolved multiple alpha (TSA) and beta (TSB) homologs, which have probably diverged in biological function and their ability of subunit interaction. There is some evidence for a tryptophan synthase (TS) complex in Arabidopsis. On the other hand maize (Zea mays) expresses the TSA-homologs BX1 and IGL that efficiently cleave IGP, independent of interaction with TSB.
resultsIn order to clarify, how tryptophan is synthesized in maize, two TSA homologs, hitherto uncharacterized ZmTSA and ZmTSAlike, were functionally analyzed. ZmTSA is localized in plastids, the major site of tryptophan biosynthesis in plants. It catalyzes the tryptophan synthase alpha-reaction (cleavage of IGP), and forms a tryptophan synthase complex with ZmTSB1 in vitro. The catalytic efficiency of the alpha-reaction is strongly enhanced upon complex formation. A 160 kD tryptophan synthase complex was partially purified from maize leaves and ZmTSA was identified as native alpha-subunit of this complex by mass spectrometry. ZmTSAlike, for which no in vitro activity was detected, is localized in the cytosol. ZmTSAlike, BX1, and IGL were not detectable in the native tryptophan synthase complex in leaves.
conclusionIt was demonstrated in vivo and in vitro that maize forms a tryptophan synthase complex and ZmTSA functions as alpha-subunit in this complex.
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