ArticleMolecular reproduction and development2009
Expression profile of male germ cell-associated genes in mouse embryonic stem cell cultures treated with all-trans retinoic acid and testosterone.
Article in Molecular reproduction and development, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 69 citations in OpenAlex.
- Associations of Bovine Fetal Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells and Germ Cell Marker Expression in Testicular Organoids.Animals : an open access journal from MDPI · 2026Article
- Retinoic acid receptor regulation of decision-making for cell differentiation.Frontiers in cell and developmental biology · 2023Review
- How does retinoic acid (RA) signaling pathway regulate spermatogenesis?Histology and histopathology · 2022Review
- Autophagy core protein ATG5 is required for elongating spermatid development, sperm individualization and normal fertility in male mice.Autophagy · 2021Article
- Primordial germ cells can be differentiated by retinoic acid and progesterone induction from embryonic stem cells.Journal of biosciences · 2021Article
- The essential role of intraflagellar transport protein IFT81 in male mice spermiogenesis and fertility.American journal of physiology. Cell physiology · 2020Article
- Murine germ cell-specific disruption of Ift172 causes defects in spermiogenesis and male fertility.Reproduction (Cambridge, England) · 2020Article
- Localized Induction of Gene Expression in Embryonic Stem Cell Aggregates Using Holographic Optical Tweezers to Create Biochemical Gradients.Regenerative engineering and translational medicine · 2020Article
- The role of microRNAs in embryonic stem cell and induced pluripotent stem cell differentiation in male germ cells.Journal of cellular physiology · 2019Review
- Indirect Co-Culture of Testicular Cells with Bone Marrow Mesenchymal Stem Cells Leads to Male Germ Cell-Specific Gene Expressions.Cell journal · 2019Article
- Combinatorial knockout of RARα, RARβ, and RARγ completely abrogates transcriptional responses to retinoic acid in murine embryonic stem cells.The Journal of biological chemistry · 2018Article
- The cell adhesion molecule BT-IgSF is essential for a functional blood-testis barrier and male fertility in mice.The Journal of biological chemistry · 2017Article
- Investigating right ovary degeneration in chick embryos by transcriptome sequencing.The Journal of reproduction and development · 2017Article
- Induced pluripotent stem cell-derived gamete-associated proteins incite rejection of induced pluripotent stem cells in syngeneic mice.Immunology · 2017Article
- CHIR99021 combined with retinoic acid promotes the differentiation of primordial germ cells from human embryonic stem cells.Oncotarget · 2017Article
- Article
- Selection of the Inducer for the Differentiation of Chicken Embryonic Stem Cells into Male Germ Cells In Vitro.PloS one · 2016Article
- Sertoli cell-mediated differentiation of male germ cell-like cells from human umbilical cord Wharton's jelly-derived mesenchymal stem cells in an in vitro co-culture system.European journal of medical research · 2015Article
- Comparative analysis of temporal gene expression patterns in the developing ovary of the embryonic chicken.The Journal of reproduction and development · 2015Article
- Cloning and expression characteristics of the pig Stra8 gene.International journal of molecular sciences · 2014Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
Cells that morphologically and functionally resemble male germ cells can be spontaneously derived from ES cells. However, this process is inefficient and unpredictable suggesting that the expression pattern of male germ cell associated genes during spontaneous ES cell differentiation does not mimic the in vivo profiles of the genes. Thus, in the present study, the temporal profile of genes expressed at different stages of male germ cell development was examined in differentiating ES cells. The effect of all-trans retinoic acid (RA) which is a known inducer of primordial germ cell (PGC) proliferation/survival in vitro and testosterone which is required for spermatogenesis in vivo on the expression of these genes was also determined. Each of the 12 genes analyzed exhibited one of four temporal expression patterns in untreated differentiating ES cells: progressively decreased (Dppa3, Sycp3, Msy2), initially low and then increased (Stra8, Sycp1, Dazl, Act, Prm1), initially decreased and then increased (Piwil2, Tex14), or relatively unchanged (Akap3, Odf2). RA-treated cells exhibited increased expression of Stra8, Dazl, Act, and Prm1 and suppressed expression of Dppa3 compared to untreated controls. Furthermore, testosterone increased expression of Stra8 while the combination of RA and testosterone synergistically increased expression of Act. Our findings establish a comprehensive profile of male germ cell gene expression during spontaneous differentiation of murine ES cells and describe the capacity of RA and testosterone to modulate the expression of these genes. Furthermore, these data represent an important first step in designing a plausible directed differentiation protocol for male germ cells.
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