ArticleMarine biotechnology (New York, N.Y.)
Cloning and use of a coral 36B4 gene to study the differential expression of coral genes between light and dark conditions.
Article in Marine biotechnology (New York, N.Y.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Selection of suitable reference genes for gene expression studies in myxosporean (Myxozoa, Cnidaria) parasites.Scientific reports · 2019Article
- Molecular cloning and characterization of calmodulin-like protein CaLP from the Scleractinian coral Galaxea astreata.Cell stress & chaperones · 2018Article
- Comparative analysis of the genomes of Stylophora pistillata and Acropora digitifera provides evidence for extensive differences between species of corals.Scientific reports · 2017Article
- Coral Carbonic Anhydrases: Regulation by Ocean Acidification.Marine drugs · 2016Article
- Molecular assessment of the effect of light and heterotrophy in the scleractinian coral Stylophora pistillata.Proceedings. Biological sciences · 2016Article
- Carbonic Anhydrases in Cnidarians: Novel Perspectives from the Octocorallian Corallium rubrum.PloS one · 2016Article
- Bicarbonate transporters in corals point towards a key step in the evolution of cnidarian calcification.Scientific reports · 2015Article
- Rubisco expression in the dinoflagellate Symbiodinium sp. is influenced by both photoperiod and endosymbiotic lifestyle.Marine biotechnology (New York, N.Y.) · 2014Article
- Article
- High adenylyl cyclase activity and in vivo cAMP fluctuations in corals suggest central physiological role.Scientific reports · 2013Article
- Cell biology of cnidarian-dinoflagellate symbiosis.Microbiology and molecular biology reviews : MMBR · 2012Review
- Thermal stress promotes host mitochondrial degradation in symbiotic cnidarians: are the batteries of the reef going to run out?PloS one · 2012Article
- A new coral carbonic anhydrase in Stylophora pistillata.Marine biotechnology (New York, N.Y.) · 2011Article
- Regulation of apoptotic mediators reveals dynamic responses to thermal stress in the reef building coral Acropora millepora.PloS one · 2011Article
- Patterns of gene expression in a scleractinian coral undergoing natural bleaching.Marine biotechnology (New York, N.Y.) · 2010Article
- Identification and expression analysis of genes involved in early ovary development in diploid gynogenetic hybrids of red crucian carp x common carp.Marine biotechnology (New York, N.Y.) · 2010Article
- Specific expression of BMP2/4 ortholog in biomineralizing tissues of corals and action on mouse BMP receptor.Marine biotechnology (New York, N.Y.)Article
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Authors and funding
7 authors.
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Abstract
This paper aims to validate reference genes for gene expression studies between light and dark conditions in the scleractinian coral Stylophora pistillata for future gene expression studies of the "light-enhanced calcification" phenomenon. For this purpose, we cloned, sequenced, and characterized a candidate reference gene, the 36B4 gene from the coral S. pistillata, and validated 36B4 and beta-actin as reference genes. To illustrate the future applications of these reference genes, we tested the dark and light expression of two photosynthetic genes (Rubisco and D1 protein of the photosystem II) and two genes encoding proteins involved in calcium transport for coral calcification (a calcium ATPase and a calcium channel). Results show that both photosynthetic genes are enhanced during the light when standardized against 36B4 and beta-actin, whereas the two genes encoding proteins involved in calcium transport are not differentially expressed between light and dark conditions. The characterization of a coral 36B4 and the establishment of such valid reference genes will be useful for future gene expression studies between diverse conditions (aposymbiotic/symbiotic, stress/control, light/dark conditions) in scleractinian corals.
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