ArticleNucleic acids research2008
Crystal structures of I-SceI complexed to nicked DNA substrates: snapshots of intermediates along the DNA cleavage reaction pathway.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- A double-strand break can trigger immunoglobulin gene conversion.Nucleic acids research · 2017Article
- Rapid prototyping of microbial cell factories via genome-scale engineering.Biotechnology advances · 2015Review
- Visualizing phosphodiester-bond hydrolysis by an endonuclease.Nature structural & molecular biology · 2015Article
- Reprogramming homing endonuclease specificity through computational design and directed evolution.Nucleic acids research · 2014Article
- The mitochondrial LSU rRNA group II intron of Ustilago maydis encodes an active homing endonuclease likely involved in intron mobility.PloS one · 2012Article
- Nucleases: diversity of structure, function and mechanism.Quarterly reviews of biophysics · 2011Review
- Homing endonucleases: from microbial genetic invaders to reagents for targeted DNA modification.Structure (London, England : 1993) · 2011Review
- Evolution of I-SceI homing endonucleases with increased DNA recognition site specificity.Journal of molecular biology · 2011Article
- Natural and engineered nicking endonucleases--from cleavage mechanism to engineering of strand-specificity.Nucleic acids research · 2011Review
- Homing endonucleases: from basics to therapeutic applications.Cellular and molecular life sciences : CMLS · 2010Review
- Generation of a nicking enzyme that stimulates site-specific gene conversion from the I-AniI LAGLIDADG homing endonuclease.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Crystal structure of I-DmoI in complex with its target DNA provides new insights into meganuclease engineering.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Engineering variants of the I-SceI homing endonuclease with strand-specific and site-specific DNA-nicking activity.Journal of molecular biology · 2008Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
I-SceI is a homing endonuclease that specifically cleaves an 18-bp double-stranded DNA. I-SceI exhibits a strong preference for cleaving the bottom strand DNA. The published structure of I-SceI bound to an uncleaved DNA substrate provided a mechanism for bottom strand cleavage but not for top strand cleavage. To more fully elucidate the I-SceI catalytic mechanism, we determined the X-ray structures of I-SceI in complex with DNA substrates that are nicked in either the top or bottom strands. The structures resemble intermediates along the DNA cleavage reaction. In a structure containing a nick in the top strand, the spatial arrangement of metal ions is similar to that observed in the structure that contains uncleaved DNA, suggesting that cleavage of the bottom strand occurs by a common mechanism regardless of whether this strand is cleaved first or second. In the structure containing a nick in the bottom strand, a new metal binding site is present in the active site that cleaves the top strand. This new metal and a candidate nucleophilic water molecule are correctly positioned to cleave the top strand following bottom strand cleavage, providing a plausible mechanism for top strand cleavage.
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Registered trials
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