ArticleThe Journal of biological chemistry2008
Bone morphogenetic protein 2 mediates dentin sialophosphoprotein expression and odontoblast differentiation via NF-Y signaling.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 115 citations in OpenAlex.
- Effect of dentin conditioners on biomarker release and microhardness of radicular dentin: an in vitro study.BMC oral health · 2026Article
- SLIT3: a novel regulator of odontogenic differentiation through Akt/GSK3β/β-catenin signaling pathway.International journal of oral science · 2026Article
- Crosstalk Between Hematopoietic and Mesenchymal Cells in Bone and Tooth Immune Response.Advances in experimental medicine and biology · 2026Review
- Regenerative Endodontic Therapies: Harnessing Stem Cells, Scaffolds, and Growth Factors.Polymers · 2025Review
- The Biofunctional Monomer, the Calcium Salt of 4-Methacryloxyethyl Trimellitic Acid, Promotes Odontoblast Differentiation in Three-Dimensional Culture System.International journal of biomaterials · 2025Article
- Animal models and related techniques for dentin study.Odontology · 2025Review
- Osteogenic Differentiation Capacity of Dental Pulp Stem Cells on 3D Printed Polyurethane/Boric Acid Scaffold.Biological trace element research · 2024Article
- Effects of different signaling pathways on odontogenic differentiation of dental pulp stem cells: a review.Frontiers in physiology · 2023Review
- The odontoblastic differentiation of dental mesenchymal stem cells: molecular regulation mechanism and related genetic syndromes.Frontiers in cell and developmental biology · 2023Review
- Review
- Dentine sialophosphoprotein signal in dentineogenesis and dentine regeneration.European cells & materials · 2021Review
- FGF8 and BMP2 mediated dynamic regulation of dental mesenchyme proliferation and differentiation via Lhx8/Suv39h1 complex.Journal of cellular and molecular medicine · 2021Article
- Differential lncRNA/mRNA Expression Profiling and Functional Network Analyses in Bmp2 Deletion of Mouse Dental Papilla Cells.Frontiers in genetics · 2021Article
- Efficacy of Photobiomodulation and Vitamin D on Odontogenic Activity of Human Dental Pulp Stem Cells.Journal of lasers in medical sciences · 2021Article
- Biomimetic Aspects of Restorative Dentistry Biomaterials.Biomimetics (Basel, Switzerland) · 2020Review
- Transit amplifying cells coordinate mouse incisor mesenchymal stem cell activation.Nature communications · 2019Article
- Mutant Dentin Sialophosphoprotein Causes Dentinogenesis Imperfecta.Journal of dental research · 2019Article
- Identification of dentinogenic cell-specific surface antigens in odontoblast-like cells derived from adult dental pulp.Stem cell research & therapy · 2019Article
- Experimental animal study on BMP-3 expression in periodontal tissues in the process of orthodontic tooth movement.Experimental and therapeutic medicine · 2019Article
- Dental pulp stem cells and osteogenesis: an update.Cytotechnology · 2018Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
Dentin sialophosphoprotein (DSPP), an important odontoblast differentiation marker, is necessary for tooth development and mineralization. Bone morphogenetic protein 2 (BMP2) plays a vital role in odontoblast function via diverse signal transduction systems. We hypothesize that BMP2 regulates DSPP gene transcription and thus odontoblast differentiation. Here we report that expression of BMP2 and DSPP is detected during mouse odontogenesis by in situ hybridization assay, and BMP2 up-regulates DSPP mRNA and protein expression as well as DSPP-luciferase promoter activity in mouse preodontoblasts. By sequentially deleting fragments of the mouse DSPP promoter, we show that a BMP2-response element is located between nucleotides -97 and -72. By using antibody and oligonucleotide competition assays in electrophoretic mobility shift analysis and chromatin immunoprecipitation experiments, we show that the heterotrimeric transcription factor Y (NF-Y) complex physically interacts with the inverted CCAAT box within the BMP2-response element. BMP2 induces NF-Y accumulation into the nucleus increasing its recruitment to the mouse DSPP promoter in vivo. Furthermore, forced overexpression of NF-Y enhances promoter activity and increases endogenous DSPP protein levels. In contrast, mutations in the NF-Y-binding motif reduce BMP2-induced DSPP transcription. Moreover, inhibiting BMP2 signaling by Noggin, a BMP2 antagonist, results in significant inhibition of DSPP gene expression in preodontoblasts. Taken together, these results indicate that BMP2 mediates DSPP gene expression and odontoblast differentiation via NF-Y signaling during tooth development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.