ArticleApplied and environmental microbiology2008
Rapid screening of quorum-sensing signal N-acyl homoserine lactones by an in vitro cell-free assay.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 89 citations in OpenAlex.
- Gene circuit-based sensors.Fundamental research · 2025Review
- Assessment of microbial antagonistic activity and Quorum Sensing Signal Molecule (Cyclopeptides-DKPs and N-Acyl Homoserine Lactones) detection in bacterial strains obtained from avocado thrips (Thysanoptera: Thripidae).Biotechnology reports (Amsterdam, Netherlands) · 2025Article
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- Impact of Graphene Layers on Genetic Expression and Regulation within Sulfate-Reducing Biofilms.Microorganisms · 2024Article
- Circular single-stranded DNA as a programmable vector for gene regulation in cell-free protein expression systems.Nature communications · 2024Article
- N-acyl homoserine lactone molecules assisted quorum sensing: effects consequences and monitoring of bacteria talking in real life.Archives of microbiology · 2021Review
- Exogenous autoinducer-2 inhibits biofilm development of Desulfovibrio sp. Huiquan2017.World journal of microbiology & biotechnology · 2021Article
- Article
- Photoluminescence-Based Bioassay With Cysteamine-Capped TiOFrontiers in bioengineering and biotechnology · 2021Article
- Gene Sets and Mechanisms of Sulfate-Reducing Bacteria Biofilm Formation and Quorum Sensing With Impact on Corrosion.Frontiers in microbiology · 2021Review
- Portable environment-signal detection biosensors with cell-free synthetic biosystems.RSC advances · 2020Article
- Degradation of BTEX mixture by a new Pseudomonas putida strain: role of the quorum sensing in the modulation of the upper BTEX oxidative pathway.Environmental science and pollution research international · 2020Article
- Engineering acyl-homoserine lactone-interfering enzymes toward bacterial control.The Journal of biological chemistry · 2020Review
- Detection of Quorum-Sensing Molecules for Pathogenic Molecules Using Cell-Based and Cell-Free Biosensors.Antibiotics (Basel, Switzerland) · 2020Review
- Cell-free gene expression: an expanded repertoire of applications.Nature reviews. Genetics · 2020Review
- A Primer on Emerging Field-Deployable Synthetic Biology Tools for Global Water Quality Monitoring.NPJ clean water · 2020Article
- Effect of Quorum Sensing on the Ability ofApplied and environmental microbiology · 2019Article
- Review
- Salinity-Mediated Increment in Sulfate Reduction, Biofilm Formation, and Quorum Sensing: A Potential Connection Between Quorum Sensing and Sulfate Reduction?Frontiers in microbiology · 2019Article
- AHLs-produced bacteria in refrigerated shrimp enhanced the growth and spoilage ability ofJournal of food science and technology · 2019Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A simple, sensitive, and rapid cell-free assay system was developed for detection of N-acyl homoserine lactone (AHL) autoinducers involved in bacterial quorum sensing (QS). The present approach improves upon previous whole-cell biosensor-based approaches in its utilization of a cell-free assay approach to conduct bioassays. The cell-free assay was derived from the AHL biosensor bacterium Agrobacterium tumefaciens NTL4(pCF218)(pCF372), allowing the expression of beta-galactosidase upon addition of exogenous AHLs. We have shown that beta-galactosidase expression is possible in cell-free solution [lysate from Agrobacterium tumefaciens NTL4(pCF218)(pCF372) culture]. Assay detection limits with the use of chromogenic substrate X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) ranged from approximately 100 nM to 300 nM depending on the specific AHL. Replacement (of X-Gal) with the luminescent substrate Beta-Glo increased sensitivity to AHLs by 10-fold. A major advantage of the cell-free assay system is elimination of time-consuming steps for biosensor cell culture conditioning, which are required prior to whole-cell bioassays. This significantly reduced assay times from greater than 24 h to less than 3 h, while maintaining high sensitivity. Assay lysate may be prepared in bulk and stored (-80 degrees C) over 6 months for future use. Finally, the present protocol may be adapted for use with other biosensor strains and be used in high-throughput AHL screening of bacteria or metagenomic libraries.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.