ArticleJournal of bacteriology2008
Transcriptional interference and repression modulate the conjugative ability of the symbiotic plasmid of Rhizobium etli.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Comparative genomic analysis ofApplied and environmental microbiology · 2026Article
- Characterization of RcgA and RcgR, two rhizobial proteins involved in the modulation of plasmid transfer.Microbiology spectrum · 2026Article
- RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial Plasmids.mBio · 2022Article
- Why are rhizobial symbiosis genes mobile?Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Article
- Transfer of the Symbiotic Plasmid ofFrontiers in microbiology · 2020Article
- DNA-Binding Proteins Regulating pIP501 Transfer and Replication.Frontiers in molecular biosciences · 2016Review
- A Functional oriT in the Ptw Plasmid of Burkholderia cenocepacia Can Be Recognized by the R388 Relaxase TrwC.Frontiers in molecular biosciences · 2016Article
- Characterization of IntA, a bidirectional site-specific recombinase required for conjugative transfer of the symbiotic plasmid of Rhizobium etli CFN42.Journal of bacteriology · 2013Article
- Genetic characterization of a novel rhizobial plasmid conjugation system in Rhizobium leguminosarum bv. viciae strain VF39SM.Journal of bacteriology · 2013Article
- Antisense RNA associated with biological regulation of a restriction-modification system.Nucleic acids research · 2011Article
- pSymA-dependent mobilization of the Sinorhizobium meliloti pSymB megaplasmid.Journal of bacteriology · 2010Article
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9 authors.
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Abstract
Bacteria of the order Rhizobiales are able to establish nitrogen-fixing symbioses with legumes. Commonly, genes for symbiosis are harbored on large symbiotic plasmids. Although the transfer of symbiotic plasmids is commonly detected in nature, there are few experimentally characterized examples. In Rhizobium etli, the product of rctA inhibits the conjugation of the symbiotic plasmid by reducing the transcription of the virB operon. rctA is transcribed divergently from this operon, and its product is predicted to have a DNA binding domain. In the present study, using DNase I footprinting and binding assays, we demonstrated the specific binding of RctA to the virB operon promoter. A 9-bp motif in the spacer region of this promoter (the rctA binding motif box) and the presence of a functional -10 region were critical elements for RctA binding. Transcriptional fusion analyses revealed that the elimination of either element provoked a relief of RctA-mediated repression. These data support a model in which RctA inhibits the access of the RNA polymerase to the virB promoter. Interestingly, rctA expression levels were modulated by transcriptional interference from transcripts emanating from the virB promoter. This phenomenon adds another level of regulation for this system, thus revealing a novel mechanism of plasmid transfer regulation in the Rhizobiales.
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