ArticleBiophysical journal2008
A fluorescence-based technique to construct size distributions from single-object measurements: application to the extrusion of lipid vesicles.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed.
- Intrinsic Fluorescence as a Probe of Structural Dynamics and Interactions of Human Adenovirus with Surfactants and Liposomes.Journal of fluorescence · 2026Article
- Laser speckle particle sizer (SPARSE) informs the size distribution of tissue granularities.Science advances · 2025Article
- Single-vesicle Tracking of α-Synuclein Oligomers Reveals Pore Formation by a Three-Stage Model.ACS nano · 2025Article
- Exploration and analytical techniques for membrane curvature-sensing proteins in bacteria.Journal of bacteriology · 2025Review
- Investigating Complexin-Membrane Interactions Using NMR and Optical Methods.Methods in molecular biology (Clifton, N.J.) · 2025Article
- A single-particle analysis method for detecting membrane remodelling and curvature sensing.Journal of cell science · 2024Article
- Multivariate Analysis of Individual Bacterial Outer Membrane Vesicles Using Fluorescence Microscopy.Chemical & biomedical imaging · 2024Article
- Deconvoluting the Effect of Cell-Penetrating Peptides for Enhanced and Controlled Insertion of Large-Scale DNA Nanopores.ACS applied materials & interfaces · 2024Article
- Dynamic structure factor of undulating vesicles: finite-size and spherical geometry effects with application to neutron spin echo experiments.The European physical journal. E, Soft matter · 2024Article
- In vitro Assay to Evaluate Cation Transport of Ionophores.Bio-protocol · 2022Article
- Molecular mechanisms of steric pressure generation and membrane remodeling by disordered proteins.Biophysical journal · 2022Article
- Single Vesicle Fluorescence-Bleaching Assay for Multi-Parameter Analysis of Proteoliposomes by Total Internal Reflection Fluorescence Microscopy.ACS applied materials & interfaces · 2022Article
- Insights into Membrane Curvature Sensing and Membrane Remodeling by Intrinsically Disordered Proteins and Protein Regions.The Journal of membrane biology · 2022Review
- Crowding-induced morphological changes in synthetic lipid vesicles determined using smFRET.Frontiers in bioengineering and biotechnology · 2022Article
- Single-vesicle intensity and colocalization fluorescence microscopy to study lipid vesicle fusion, fission, and lipid exchange.Frontiers in molecular neuroscience · 2022Article
- Nanoarrays of Individual Liposomes and Bacterial Outer Membrane Vesicles by Liftoff Nanocontact Printing.Small (Weinheim an der Bergstrasse, Germany) · 2021Article
- Clathrin senses membrane curvature.Biophysical journal · 2021Article
- Lipid Composition Affects the Efficiency in the Functional Reconstitution of the CytochromeInternational journal of molecular sciences · 2020Article
- How Membrane Geometry Regulates Protein Sorting Independently of Mean Curvature.ACS central science · 2020Article
- Protons in small spaces: Discrete simulations of vesicle acidification.PLoS computational biology · 2019Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report a novel approach to quantitatively determine complete size distributions of surface-bound objects using fluorescence microscopy. We measure the integrated intensity of single particles and relate it to their size by taking into account the object geometry and the illumination profile of the microscope, here a confocal laser scanning microscope. Polydisperse (as well as monodisperse) size distributions containing objects both below and above the optical resolution of the microscope are recorded and analyzed. The data is collected online within minutes, which allows the user to correlate the size of an object with the response from any given fluorescence-based biochemical assay. We measured the mean diameter of extruded fluorescently labeled lipid vesicles using the proposed method, dynamic light scattering, and cryogenic transmission electron microscopy. The three techniques were in excellent agreement, measuring the same values within 7-9%. Furthermore we demonstrated here, for the first time that we know of, the ability to determine the full size distribution of polydisperse samples of nonextruded lipid vesicles. Knowledge of the vesicle size distribution before and after extrusion allowed us to propose an empirical model to account for the effect of extrusion on the complete size distribution of vesicle samples.
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