ArticleBiophysical journal2008
DNA overstretching in the presence of glyoxal: structural evidence of force-induced DNA melting.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 83 citations in OpenAlex.
- Force-induced melting and S-DNA pathways for DNA overstretching exhibit distinct kinetics.Nucleic acids research · 2025Article
- Structure and Dynamics of dsDNA in Cell-like Environments.Entropy (Basel, Switzerland) · 2022Review
- Article
- Single-molecule mechanochemical characterization of E. coli pol III core catalytic activity.Protein science : a publication of the Protein Society · 2017Article
- Single-molecule studies of high-mobility group B architectural DNA bending proteins.Biophysical reviews · 2017Review
- Mechanisms of small molecule-DNA interactions probed by single-molecule force spectroscopy.Nucleic acids research · 2016Article
- Dynamic Release of Bending Stress in Short dsDNA by Formation of a Kink and Forks.Angewandte Chemie (International ed. in English) · 2015Article
- Single aromatic residue location alters nucleic acid binding and chaperone function of FIV nucleocapsid protein.Virus research · 2014Article
- Force-induced melting of DNA--evidence for peeling and internal melting from force spectra on short synthetic duplex sequences.Nucleic acids research · 2014Article
- Transient kinetics measured with force steps discriminate between double-stranded DNA elongation and melting and define the reaction energetics.Nucleic acids research · 2014Article
- Differential contribution of basic residues to HIV-1 nucleocapsid protein's nucleic acid chaperone function and retroviral replication.Nucleic acids research · 2014Article
- DNA stretching on the wall surfaces in curved microchannels with different radii.Nanoscale research letters · 2014Article
- An effective mesoscopic model of double-stranded DNA.Journal of biological physics · 2014Article
- Global force-torque phase diagram for the DNA double helix: structural transitions, triple points, and collapsed plectonemes.Physical review. E, Statistical, nonlinear, and soft matter physics · 2013Article
- Polymerase manager protein UmuD directly regulates Escherichia coli DNA polymerase III α binding to ssDNA.Nucleic acids research · 2013Article
- Revealing the competition between peeled ssDNA, melting bubbles, and S-DNA during DNA overstretching using fluorescence microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Aromatic residue mutations reveal direct correlation between HIV-1 nucleocapsid protein's nucleic acid chaperone activity and retroviral replication.Virus research · 2013Article
- Single-molecule kinetics reveal microscopic mechanism by which High-Mobility Group B proteins alter DNA flexibility.Nucleic acids research · 2013Article
- Tension induces a base-paired overstretched DNA conformation.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Two distinct overstretched DNA structures revealed by single-molecule thermodynamics measurements.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
When a long DNA molecule is stretched beyond its B-form contour length, a transition occurs in which its length increases by a factor of 1.7, with very little force increase. A quantitative model was proposed to describe this transition as force-induced melting, where double-stranded DNA is converted into single-stranded DNA. The force-induced melting model accurately describes the thermodynamics of DNA overstretching as a function of solution conditions and in the presence of DNA binding ligands. An alternative explanation suggests a transformation into S-DNA, a double-stranded form which preserves the interstrand base pairing. To determine the extent to which DNA base pairs are exposed to solution during the transition, we held DNA overstretched to different lengths within the transition in the presence of glyoxal. If overstretching involved strand separation, then force-melted basepairs would be glyoxal-modified, thus essentially permanently single-stranded. Subsequent stretches confirm that a significant fraction of the DNA melted by force is permanently melted. This result demonstrates that DNA overstretching is accompanied by a disruption of the DNA helical structure, including a loss of hydrogen bonding.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.