ArticleBiophysical journal2008
Mitochondrial swelling measurement in situ by optimized spatial filtering: astrocyte-neuron differences.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 67 citations in OpenAlex.
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- A Fully-Automated Senescence Test (FAST) for the high-throughput quantification of senescence-associated markers.GeroScience · 2024Article
- Single-cell analysis identifies conserved features of immune dysfunction in simulated microgravity and spaceflight.Nature communications · 2024Article
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- GPR84-mediated signal transduction affects metabolic function by promoting brown adipocyte activity.The Journal of clinical investigation · 2023Article
- Milton assembles large mitochondrial clusters, mitoballs, to sustain spermatogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Mitochondrial Bioenergy in Neurodegenerative Disease: Huntington and Parkinson.International journal of molecular sciences · 2023Review
- Respiratory pathology in the OptnRespiratory physiology & neurobiology · 2020Article
- Motor axonopathies in a mouse model of Duchenne muscular dystrophy.Scientific reports · 2020Article
- Preeclampsia with Intrauterine Growth Restriction Generates Morphological Changes in Endothelial Cells Associated with Mitochondrial Swelling-An In Vitro Study.Journal of clinical medicine · 2019Article
- Guidelines for evaluating myocardial cell death.American journal of physiology. Heart and circulatory physiology · 2019Review
- Oxidative Phosphorylation Impairment by DDT and DDE.Frontiers in endocrinology · 2019Review
- Natural Genetic Variation in Yeast Reveals That NEDD4 Is a Conserved Modifier of Mutant Polyglutamine Aggregation.G3 (Bethesda, Md.) · 2018Article
- Mitochondrial toxicity induced by a thiourea gold(i) complex: mitochondrial permeability transition and respiratory deficit.Toxicology research · 2018Article
- Computational Modeling of In Vitro Swelling of Mitochondria: A Biophysical Approach.Molecules (Basel, Switzerland) · 2018Article
- Mitochondrial toxicity of organic arsenicals: membrane permeability transition pore opening and respiratory dysfunction.Toxicology research · 2018Article
- Secondary Degeneration of Auditory Neurons after Topical Aminoglycoside Administration in a Gerbil Model.BioMed research international · 2018Article
- Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Mitochondrial swelling is a hallmark of mitochondrial dysfunction, and is an indicator of the opening of the mitochondrial permeability transition pore. We introduce here a novel quantitative in situ single-cell assay of mitochondrial swelling based on standard wide-field or confocal fluorescence microscopy. This morphometric technique quantifies the relative diameter of mitochondria labeled by targeted fluorescent proteins. Fluorescence micrographs are spatial bandpass filtered transmitting either high or low spatial frequencies. Mitochondrial swelling is measured by the fluorescence intensity ratio of the high- to low-frequency filtered copy of the same image. We have termed this fraction the "thinness ratio". The filters are designed by numeric optimization for sensitivity. We characterized the thinness ratio technique by modeling microscopic image formation and by experimentation in cultured cortical neurons and astrocytes. The frequency domain image processing endows robustness and subresolution sensitivity to the thinness ratio technique, overcoming the limitations of shape measurement approaches. The thinness ratio proved to be highly sensitive to mitochondrial swelling, but insensitive to fission or fusion of mitochondria. We found that in situ astrocytic mitochondria swell upon short-term uncoupling or inhibition of oxidative phosphorylation, whereas such responses are absent in cultured cortical neurons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.