ArticleNeuroscience2008
Identification and immunohistochemical characterization of colospinal afferent neurons in the rat.
Article in Neuroscience, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 21 citations in OpenAlex.
- Gut-brain communication: types of sensory nerves and mechanisms of activation.Nature reviews. Gastroenterology & hepatology · 2026Review
- 5-HTThe Journal of physiology · 2025Article
- Protease-activated receptors in health and disease.Physiological reviews · 2023Review
- Changes in nitrergic and tachykininergic pathways in rat proximal colon in response to chronic treatment with otilonium bromide.Neurogastroenterology and motility · 2015Article
- Transient receptor potential vanilloid type 1 channel (TRPV1) immunolocalization in the murine enteric nervous system is affected by the targeted C-terminal epitope of the applied antibody.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2013Article
- In vitro and in vivo investigations on the effects of low-density lipoprotein concentration polarization and haemodynamics on atherosclerotic localization in rabbit and zebrafish.Journal of the Royal Society, Interface · 2013Article
- Extrinsic primary afferent signalling in the gut.Nature reviews. Gastroenterology & hepatology · 2013Review
- Lesioning of TRPV1 expressing primary afferent neurons prevents PAR-2 induced motility, but not mechanical hypersensitivity in the rat colon.Neurogastroenterology and motility · 2012Article
- Hyperpolarization-activated cyclic nucleotide-gated cation channel subtypes differentially modulate the excitability of murine small intestinal afferents.World journal of gastroenterology · 2012Article
- Protease-activated receptor 2 signaling in inflammation.Seminars in immunopathology · 2012Review
- Transient receptor potential (TRP) channels as drug targets for diseases of the digestive system.Pharmacology & therapeutics · 2011Review
- Characterization of silent afferents in the pelvic and splanchnic innervations of the mouse colorectum.American journal of physiology. Gastrointestinal and liver physiology · 2011Article
- TRP channels in the digestive system.Current pharmaceutical biotechnology · 2011Review
- Visceral organ cross-sensitization - an integrated perspective.Autonomic neuroscience : basic & clinical · 2010Review
- Cystitis increases colorectal afferent sensitivity in the mouse.American journal of physiology. Gastrointestinal and liver physiology · 2009Article
- NMDA receptor subunit expression and PAR2 receptor activation in colospinal afferent neurons (CANs) during inflammation induced visceral hypersensitivity.Molecular pain · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The classification, morphology and function of enteric neurons have been extensively studied in the small and large intestine. However, little is known about enteric neurons that directly project to the CNS. Previous studies have identified these unique neurons in the rectum, rectospinal neurons, but little was done to characterize them. Therefore, the aim of this study was to identify and characterize enteric neurons in the rat colon that directly project to the CNS by using retrograde neuronal tracing and immunohistochemistry. By applying the retrograde tracers 1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanine perchlorate (DiI) and Fluorogold (FG) to the L6/S1 segments of the spinal cord, we identified these neurons in both the myenteric and submucosal plexuses of the colon. These neurons were immunoreactive for neurofilament (NF) a marker for Adelta-fibers and isolectin-B4 (IB(4)) a marker for C-fibers. These neurons expressed the enzyme neuronal nitric oxide synthase (nNOS) as well as peptides associated with sensory neurons such as substance P (SP) and vasoactive intestinal polypeptide (VIP) but did not express calcitonin gene-related peptide (CGRP). The N-methyl-D-aspartate (NMDA) receptor subunits NR1 and NR2D and proteinase-activated receptor-2 (PAR2) were also found in these neurons. However they did not express the transient receptor potential receptor V1 (TRPV1) or neurokinin 1 receptor (NK1). The expression of the peptides and receptors suggests that there are at least two separate populations of neurons projecting from the colon to the CNS. The data suggest that these colospinal afferent neurons (CANs) might be involved in nociception. Whether sensory information from CANs is perceived by the animal or is part of the parasympathetic reflex is currently not known.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.