ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2008
Evaluation of Pyrosequencing technology for the identification of clinically relevant non-dematiaceous yeasts and related species.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Identification ofCurrent medical mycology · 2017Article
- Biographical Feature: Patrick R. Murray, Ph.D.Journal of clinical microbiology · 2016Article
- Rapid identification of medically important Candida isolates using high resolution melting analysis.PloS one · 2015Article
- Digestive fungal flora in asymptomatic subjects in Bobo-Dioulasso, Burkina Faso.Asian Pacific journal of tropical biomedicine · 2014Article
- Application of the novel method in the diagnosis and treatment of median rhomboid glossitis Candida-associated.European journal of dentistry · 2014Article
- Molecular identification and antifungal susceptibility testing of clinical isolates of the Candida rugosa species complex and proposal of the new species Candida neorugosa.Journal of clinical microbiology · 2012Article
- Evaluation of the commercial rapid trehalose test (GLABRATA RTT) for the point of isolation identification of Candida glabrata isolates in primary cultures.Mycopathologia · 2012Article
- Rapid identification of yeasts from positive blood culture bottles by pyrosequencing.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2011Article
- Rapid molecular identification of pathogenic yeasts by pyrosequencing analysis of 35 nucleotides of internal transcribed spacer 2.Journal of clinical microbiology · 2010Article
- Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of clinically important yeast species.Journal of clinical microbiology · 2010Article
- Matrix-assisted laser desorption ionization-time of flight mass spectrometry for fast and reliable identification of clinical yeast isolates.Journal of clinical microbiology · 2009Article
- Utility of pyrosequencing in identifying bacteria directly from positive blood culture bottles.Journal of clinical microbiology · 2009Article
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Authors and funding
7 authors.
Funding
Abstract
Pyrosequencing was used to identify 133 isolates of clinically relevant non-dematiaceous yeasts. These included 97 ATCC strains (42 type strains), seven UAMH strains, and 29 clinical isolates. Isolates belonged to the following genera: Candida (18 species), Trichosporon (10), Cryptococcus (7), Malassezia (3), Rhodotorula (2), Geotrichum (1), Blastoschizomyces (1), and Kodamaea (1). Amplicons of a hyper-variable ITS region were obtained and analyzed using Pyrosequencing technology. The data were evaluated by a BLAST search against the GenBank database and correlated with data obtained by conventional cycle sequencing of the ITS1-5.8S-ITS2 region. Cycle sequencing identified 78.9% of the isolates to the species level. Pyrosequencing technology identified 69.1%. In 90.1% of all of the strains tested, the identification results of both sequencing methods were identical. Most Candida isolates can be identified to the species level by Pyrosequencing. Trichosporon species and some Cryptococcus species cannot be differentiated at the species level. Pyrosequencing can be used for the reliable identification of most commonly isolated non-dematiaceous yeasts, with a reduction of cost per identification compared to conventional sequencing.
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