ArticleMatrix biology : journal of the International Society for Matrix Biology2008
Assay to mechanically tune and optically probe fibrillar fibronectin conformations from fully relaxed to breakage.
Article in Matrix biology : journal of the International Society for Matrix Biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
52 citing papers in PubMed, 116 citations in OpenAlex.
- Translational Extracellular Matrix Spray-Coating Approach for Large-Scale Bioactive Surface Functionalization and Tissue Repair.Advanced healthcare materials · 2025Article
- Measuring the biomechanical properties of cell-derived fibronectin fibrils.Biomechanics and modeling in mechanobiology · 2025Article
- Cell-Free Nonequilibrium Assembly for Hierarchical Protein/Peptide Nanopillars.Journal of the American Chemical Society · 2024Article
- Engineered Biomimetic Fibrillar Fibronectin Matrices Regulate Cell Adhesion Initiation, Migration, and Proliferation via α5β1 Integrin and Syndecan-4 Crosstalk.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Anastellin impacts on the processing of extracellular matrix fibronectin and stimulates release of cytokines from coronary artery smooth muscle cells.Scientific reports · 2022Article
- Review
- Effects of substrate stiffness and actin velocity on in silico fibronectin fibril morphometry and mechanics.PloS one · 2021Article
- The Janus Role of Adhesion in Chondrogenesis.International journal of molecular sciences · 2020Article
- Application of FRET Biosensors in Mechanobiology and Mechanopharmacological Screening.Frontiers in bioengineering and biotechnology · 2020Review
- Engineered Fibrillar Fibronectin Networks as Three-Dimensional Tissue Scaffolds.Advanced materials (Deerfield Beach, Fla.) · 2019Article
- Fibrillar fibronectin plays a key role as nucleator of collagen I polymerization during macromolecular crowding-enhanced matrix assembly.Biomaterials science · 2019Article
- Fibronectin fiber creep under constant force loading.Acta biomaterialia · 2019Article
- Production-scale fibronectin nanofibers promote wound closure and tissue repair in a dermal mouse model.Biomaterials · 2018Article
- Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation.Journal of visualized experiments : JoVE · 2018Article
- Tensile forces drive a reversible fibroblast-to-myofibroblast transition during tissue growth in engineered clefts.Science advances · 2018Article
- Novel peptide probes to assess the tensional state of fibronectin fibers in cancer.Nature communications · 2017Article
- Endothelium-derived fibronectin regulates neonatal vascular morphogenesis in an autocrine fashion.Angiogenesis · 2017Article
- Measuring the Poisson's Ratio of Fibronectin Using Engineered Nanofibers.Scientific reports · 2017Article
- Stretch-dependent changes in molecular conformation in fibronectin nanofibers.Biomaterials science · 2017Article
- Detection of an Integrin-Binding Mechanoswitch within Fibronectin during Tissue Formation and Fibrosis.ACS nano · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
In response to growing needs for quantitative biochemical and cellular assays that address whether the extracellular matrix (ECM) acts as a mechanochemical signal converter to co-regulate cellular mechanotransduction processes, a new assay is presented where plasma fibronectin fibers are manually deposited onto elastic sheets, while force-induced changes in protein conformation are monitored by fluorescence resonance energy transfer (FRET). Fully relaxed assay fibers can be stretched at least 5-6 fold, which involves Fn domain unfolding, before the fibers break. In native fibroblast ECM, this full range of stretch-regulated conformations coexists in every field of view confirming that the assay fibers are physiologically relevant model systems. Since alterations of protein function will directly correlate with their extension in response to force, the FRET vs. strain curves presented herein enable the mapping of fibronectin strain distributions in 2D and 3D cell cultures with high spatial resolution. Finally, cryptic sites for fibronectin's N-terminal 70-kD fragment were found to be exposed at relatively low strain, demonstrating the assay's potential to analyze stretch-regulated protein-protein interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.