Evidence map›Paper›PMID 18414625›Full record

ArticleEnvironmental health perspectives2008

Contamination of potable water distribution systems by multiantimicrobial-resistant enterohemorrhagic Escherichia coli.

Siya Ram, Poornima Vajpayee, Rishi Shanker

Abstract read
In one paragraph

Article in Environmental health perspectives, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. A One Health Review of Community-Acquired Antimicrobial-ResistantInternational journal of environmental research and public health · 2021
    Pooled it
  2. Article
  3. Molecular Diagnostic Tools Applied for Assessing Microbial Water Quality.International journal of environmental research and public health · 2022
    Review
  4. Review
  5. Article
  6. Article
  7. Bio-fabrication of silver nanoparticles byIET nanobiotechnology · 2018
    Article
  8. Article
  9. Article
  10. Article
  11. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Siya RamEnvironmental Microbiology Division, Industrial Toxicology Research Centre, Mahatma Gandhi Marg, Lucknow, India.
Poornima Vajpayee
Rishi Shanker

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe contamination of processed or unprocessed drinking water by fecal coliform bacteria has been reported worldwide. Despite a high incidence of waterborne diseases, entero-hemorrhagic Escherichia coli (EHEC) is an underacknowledged pathogen of concern to public health in India. Although the presence of EHEC is recorded in surface water resources of India, drinking water sources are yet to be investigated.

objectivesThe goal of this study was to analyze potable water samples for the presence of virulence determinants of EHEC and to determine the sensitivity of the virulence determinants to antimicrobials.

methodsWe enumerated coliform bacteria in potable water samples collected from six locations in Lucknow, a major city in northern India, using the most probable number method. E. coli (n = 81), randomly isolated by membrane-filtration technique from four sites, were identified by biochemical characterization. E. coli were not detected in samples from two other sites. We screened 15 randomly selected isolates from each site for virulence determinants of EHEC using polymerase chain reaction (PCR). The isolates positive for virulence determinants (n = 18) were screened for sensitivity to 15 antimicrobials by the disk diffusion method.

resultsBoth stx1 and stx2 genes were present in 33.3% of isolates, whereas others possessed either stx1 (11.1%) or stx2 (55.6%). eaeA, hlyA, and chuA genes were present in 100, 23.3, and 16.7% of isolates, respectively. Resistance to multiple antimicrobials was observed in potential EHEC.

conclusionsThe occurrence of multiantimicrobial-resistant EHEC in potable water is an important health concern because of the risk of waterborne outbreaks.

Indexed as

Drug Resistance, Multiple, BacterialWater MicrobiologyAdhesins, BacterialAnti-Infective AgentsBacterial Outer Membrane ProteinsEnterohemorrhagic Escherichia coliEscherichia coli ProteinsHemolysin ProteinsIndiaReceptors, Cell SurfaceShiga Toxin 1Shiga Toxin 2VirulenceWater SupplyAdhesins, BacterialAnti-Infective AgentsBacterial Outer Membrane ProteinsChuA protein, E colieaeA protein, E coliEscherichia coli ProteinsHemolysin ProteinsHlya protein, E coliReceptors, Cell SurfaceShiga Toxin 1Shiga Toxin 2drinking waterenterohemorrhagic Escherichia colimultiantimicrobial resistantvirulence determinants

Identifiers

PMID18414625
PMCPMC2290977

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.