ArticleMolecular systems biology2008
Synthetic gene brushes: a structure-function relationship.
Article in Molecular systems biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 44 citations in OpenAlex.
- Cell-free immuno-profiling on a genetically programmed biochip.Nature nanotechnology · 2026Article
- Toward Memory in a DNA Brush: Site-Specific Recombination Responsive to Polymer Density, Orientation, and Conformation.Journal of the American Chemical Society · 2023Article
- Cell-Free Gene Expression from DNA Brushes.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Boundary-Free Ribosome Compartmentalization by Gene Expression on a Surface.ACS synthetic biology · 2021Article
- From deterministic to fuzzy decision-making in artificial cells.Nature communications · 2020Article
- DNA condensation in one dimension.Nature nanotechnology · 2016Article
- Entropy-driven collective interactions in DNA brushes on a biochip.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Cell-free protein synthesis and assembly on a biochip.Nature nanotechnology · 2012Article
- Downstream DNA tension regulates the stability of the T7 RNA polymerase initiation complex.Biophysical journal · 2011Article
- Study of messenger RNA inactivation and protein degradation in an Escherichia coli cell-free expression system.Journal of biological engineering · 2010Article
- Compartmentalization by directional gene expression.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present the assembly of gene brushes by means of a photolithographic approach that allows us to control the density of end-immobilized linear double-stranded DNA polymers coding for entire genes. For 2 kbp DNAs, the mean distance varies from 300 nm, where DNAs are dilute and assume relaxed conformations, down to 30 nm, where steric repulsion at dense packing forces stretching out. We investigated the gene-to-protein relationship of firefly luciferase under the T7/E.Coli-extract expression system, as well as transcription-only reactions with T7 RNA polymerase, and found both systems to be highly sensitive to brush density, conformation, and orientation. A 'structure-function' picture emerges in which extension of genes induced by moderate packing exposes coding sequences and improves their interaction with the transcription/translation machinery. However, tighter packing impairs the penetration of the machinery into the brush. The response of expression to two-dimensional gene crowding at the nanoscale identifies gene brushes as basic controllable units en route to multicomponent synthetic systems. In turn, these brushes could deepen our understanding of biochemical reactions taking place under confinement and molecular crowding in living cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.