ArticleGenes & development2008
Translation of ASH1 mRNA is repressed by Puf6p-Fun12p/eIF5B interaction and released by CK2 phosphorylation.
Article in Genes & development, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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Who cites it
68 citing papers in PubMed, 104 citations in OpenAlex.
- Cyclin CLB2 mRNA localization and protein synthesis link cell cycle progression to bud growth.Nature communications · 2025Article
- Is there a localized role for translational quality control?RNA (New York, N.Y.) · 2023Article
- Fusarium oxysporum f. sp.mBio · 2023Article
- Actin dynamics in protein homeostasis.Bioscience reports · 2022Review
- Intracellular mRNA transport and localized translation.Nature reviews. Molecular cell biology · 2021Review
- Phosphorylation of mRNA-Binding Proteins Puf1 and Puf2 by TORC2-Activated Protein Kinase Ypk1 Alleviates Their Repressive Effects.Membranes · 2021Article
- Established and Emerging Regulatory Roles of Eukaryotic Translation Initiation Factor 5B (eIF5B).Frontiers in genetics · 2021Review
- Plant 3' Regulatory Regions From mRNA-Encoding Genes and Their Uses to Modulate Expression.Frontiers in plant science · 2020Review
- Translational regulation of protrusion-localized RNAs involves silencing and clustering after transport.eLife · 2019Article
- Translation factor mRNA granules direct protein synthetic capacity to regions of polarized growth.The Journal of cell biology · 2019Article
- Functional and molecular characterization of the conserved Arabidopsis PUMILIO protein, APUM9.Plant molecular biology · 2019Article
- Translational regulation in response to stress in Saccharomyces cerevisiae.Yeast (Chichester, England) · 2019Review
- The PUF Protein Family: Overview on PUF RNA Targets, Biological Functions, and Post Transcriptional Regulation.International journal of molecular sciences · 2018Review
- Article
- Messenger RNA transport in the opportunistic fungal pathogen Candida albicans.Current genetics · 2017Review
- Binding of DEAD-box helicase Dhh1 to the 5'-untranslated region ofThe Journal of biological chemistry · 2017Article
- Identification and characterization of roles for Puf1 and Puf2 proteins in the yeast response to high calcium.Scientific reports · 2017Article
- Post-translational modification directs nuclear and hyphal tip localization of Candida albicans mRNA-binding protein Slr1.Molecular microbiology · 2017Article
- Review
- Translational control of gurken mRNA in Drosophila development.Cell cycle (Georgetown, Tex.) · 2017Article
8 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Translational repression during mRNA transport is essential for spatial restriction of protein production. In the yeast Saccharomyces cerevisae, silencing of ASH1 mRNA before it is localized to the bud cortex in late anaphase is critical for asymmetric segregation of Ash1p to the daughter cell nucleus. Puf6p, an ASH1 mRNA-binding protein, has been implicated in this process as a translational repressor, but the underlying mechanism is unknown. Here, we used yeast extract-based in vitro translation assays, which recapitulate translation and phosphorylation, to characterize the mechanism of Puf6p-mediated translational regulation. We report that Puf6p interferes with the conversion of the 48S complex to the 80S complex during initiation, and this repression by Puf6p is mediated through the general translation factor eIF5B (Fun12p in S. cerevisiae). Puf6p interacts with Fun12p via the PUF domain, and this interaction is RNA-dependent and essential for translational repression by Puf6p. This repression is relieved by phosphorylation of the N-terminal region of Puf6p mediated by protein kinase CK2 (casein kinase II). Inhibition of phosphorylation at Ser31, Ser34, and Ser35 of Puf6p increases its translational repression and results in ASH1 mRNA delocalization. Our results indicate that Puf6p suppresses the translation initiation of ASH1 mRNA via interaction with Fun12p during its transport, and this repression can be released by CK2 phosphorylation in the N-terminal region of Puf6p when the mRNA reaches the bud tip.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.