ArticleProceedings of the National Academy of Sciences of the United States of America2008
Identification and evolution of fungal mitochondrial tyrosyl-tRNA synthetases with group I intron splicing activity.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- Insights into intraspecific variation and genotyping ofIMA fungus · 2026Article
- The Constructive Neutral Evolution of Behaviour.Ecology and evolution · 2025Article
- Group I Intron as a Potential Target for Antifungal Compounds: Development of aMolecules (Basel, Switzerland) · 2023Article
- Review
- Structural Divergence of the Group I Intron Binding Surface in Fungal Mitochondrial Tyrosyl-tRNA Synthetases That Function in RNA Splicing.The Journal of biological chemistry · 2016Article
- Evolution of RNA-protein interactions: non-specific binding led to RNA splicing activity of fungal mitochondrial tyrosyl-tRNA synthetases.PLoS biology · 2014Article
- Nuclear group I introns in self-splicing and beyond.Mobile DNA · 2013Article
- Sequencing of mitochondrial genomes of nine Aspergillus and Penicillium species identifies mobile introns and accessory genes as main sources of genome size variability.BMC genomics · 2012Article
- Yeast mitochondrial leucyl-tRNA synthetase CP1 domain has functionally diverged to accommodate RNA splicing at expense of hydrolytic editing.The Journal of biological chemistry · 2012Article
- Peptides from aminoacyl-tRNA synthetases can cure the defects due to mutations in mt tRNA genes.Mitochondrion · 2011Article
- Evaluating target silencing by short hairpin RNA mediated by the group I intron in cultured mammalian cells.BMC biotechnology · 2011Article
- NMR Structure of the C-terminal domain of a tyrosyl-tRNA synthetase that functions in group I intron splicing.Biochemistry · 2011Article
- Evolution of introns in the archaeal world.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- The scenario on the origin of translation in the RNA world: in principle of replication parsimony.Biology direct · 2010Article
- Protein roles in group I intron RNA folding: the tyrosyl-tRNA synthetase CYT-18 stabilizes the native state relative to a long-lived misfolded structure without compromising folding kinetics.Journal of molecular biology · 2010Article
- Processing of multiple-intron-containing pretRNA.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- A phage RNA-binding protein binds to a non-cognate structured RNA and stabilizes its core structure.Biochemical and biophysical research communications · 2009Article
- Toward predicting self-splicing and protein-facilitated splicing of group I introns.RNA (New York, N.Y.) · 2008Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The bifunctional Neurospora crassa mitochondrial tyrosyl-tRNA synthetase (CYT-18 protein) both aminoacylates mitochondrial tRNA(Tyr) and acts as a structure-stabilizing splicing cofactor for group I introns. Previous studies showed that CYT-18 has distinct tRNA(Tyr) and group I intron-binding sites, with the latter formed by three small "insertions" in the nucleotide-binding fold and other structural adaptations compared with nonsplicing bacterial tyrosyl-tRNA synthetases. Here, analysis of genomic sequences shows that mitochondrial tyrosyl-tRNA synthetases with structural adaptations similar to CYT-18's are uniquely characteristic of fungi belonging to the subphylum Pezizomycotina, and biochemical assays confirm group I intron splicing activity for the enzymes from several of these organisms, including Aspergillus nidulans and the human pathogens Coccidioides posadasii and Histoplasma capsulatum. By combining multiple sequence alignments with a previously determined cocrystal structure of a CYT-18/group I intron RNA complex, we identify conserved features of the Pezizomycotina enzymes related to group I intron and tRNA interactions. Our results suggest that mitochondrial tyrosyl-tRNA synthetases with group I intron splicing activity evolved during or after the divergence of the fungal subphyla Pezizomycotina and Saccharomycotina by a mechanism involving the concerted differentiation of preexisting protein loop regions. The unique group I intron splicing activity of these fungal enzymes may provide a new target for antifungal drugs.
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