ArticleBiochemical and biophysical research communications2008
ERK activation is only one role of PKC in TCR-independent cytotoxic T cell granule exocytosis.
Article in Biochemical and biophysical research communications, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 10 citations in OpenAlex.
- LPAFrontiers in immunology · 2019Article
- A high-throughput phenotypic screen of cytotoxic T lymphocyte lytic granule exocytosis reveals candidate immunosuppressants.Journal of biomolecular screening · 2015Article
- Flow cytometry enables a high-throughput homogeneous fluorescent antibody-binding assay for cytotoxic T cell lytic granule exocytosis.Journal of biomolecular screening · 2013Article
- Ghrelin directly stimulates glucagon secretion from pancreatic alpha-cells.Molecular endocrinology (Baltimore, Md.) · 2011Article
- No specific subcellular localization of protein kinase C is required for cytotoxic T cell granule exocytosis.The Journal of biological chemistry · 2009Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Cytotoxic T cells (CTLs) kill target cells by releasing lytic agents via regulated exocytosis. Three signals are known to be required for exocytosis: an increase in intracellular Ca(2+), activation of protein kinase C (PKC) and activation of extracellular signal regulated signal kinase (ERK). ERK activation required for exocytosis depends on activity of PKC. The simplest possibility is that the sole effect of PKC required for exocytosis is ERK activation. Testing this requires dissociating ERK and PKC activation. We did this using TCR-independent stimulation of TALL-104 human leukemic CTLs. When cells are stimulated with thapsigargin and PMA, agents that increase intracellular Ca(2+) and activate PKC, respectively, PKC-dependent ERK activation is required for lytic granule exocytosis. Expressing a constitutively active mutant MAP kinase kinase activates ERK independent of PKC. However, activating ERK without PKC does not support lytic granule exocytosis, indicating that there are multiple effects of PKC required for granule exocytosis.
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Registered trials
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