ArticleBMC genomics2008
Multiway real-time PCR gene expression profiling in yeast Saccharomyces cerevisiae reveals altered transcriptional response of ADH-genes to glucose stimuli.
Article in BMC genomics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 57 citations in OpenAlex.
- Multi-omics elucidation of resource allocation for enhanced ethanol production via precise glucose control in anaerobicSynthetic and systems biotechnology · 2026Article
- FUS-DDIT3 Fusion Oncoprotein Expression Affects JAK-STAT Signaling in Myxoid Liposarcoma.Frontiers in oncology · 2022Article
- Comparing the Effects of Different Unsaturated Fatty Acids on Fermentation Performance ofMolecules (Basel, Switzerland) · 2019Article
- Identification of inhibitors regulating cell proliferation and FUS-DDIT3 expression in myxoid liposarcoma using combined DNA, mRNA, and protein analyses.Laboratory investigation; a journal of technical methods and pathology · 2018Article
- Analysis of the NCR Mechanisms inFrontiers in genetics · 2018Article
- Regulation of crucial enzymes and transcription factors on 2-phenylethanol biosynthesis via Ehrlich pathway in Saccharomyces cerevisiae.Journal of industrial microbiology & biotechnology · 2017Article
- Experimental Disc Herniation in the Rat Causes Downregulation of Serotonin Receptor 2c in a TNF-dependent Manner.Clinical orthopaedics and related research · 2015Article
- Histamine promotes the development of monocyte-derived dendritic cells and reduces tumor growth by targeting the myeloid NADPH oxidase.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Invertase Suc2-mediated inulin catabolism is regulated at the transcript level in Saccharomyces cerevisiae.Microbial cell factories · 2015Article
- Selection of reference genes for quantitative real time RT-PCR during dimorphism in the zygomycete Mucor circinelloides.Molecular biology reports · 2015Article
- Non-additive transcriptional profiles underlie dikaryotic superiority in Pleurotus ostreatus laccase activity.PloS one · 2013Article
- Analysis of region-specific changes in gene expression upon treatment with citalopram and desipramine reveals temporal dynamics in response to antidepressant drugs at the transcriptome level.Psychopharmacology · 2012Article
- Article
- A novel strategy for selection and validation of reference genes in dynamic multidimensional experimental design in yeast.PloS one · 2012Article
- Defining cell populations with single-cell gene expression profiling: correlations and identification of astrocyte subpopulations.Nucleic acids research · 2011Article
- High throughput nano-liter RT-qPCR to classify soil contamination using a soil arthropod.BMC molecular biology · 2011Article
- Article
- Natural variation explains most transcriptomic changes among maize plants of MON810 and comparable non-GM varieties subjected to two N-fertilization farming practices.Plant molecular biology · 2010Article
- Validation of reference genes for quantitative expression analysis by real-time RT-PCR in Saccharomyces cerevisiae.BMC molecular biology · 2009Article
Corrections and comments
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Authors and funding
6 authors at 6 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe large sensitivity, high reproducibility and essentially unlimited dynamic range of real-time PCR to measure gene expression in complex samples provides the opportunity for powerful multivariate and multiway studies of biological phenomena. In multiway studies samples are characterized by their expression profiles to monitor changes over time, effect of treatment, drug dosage etc. Here we perform a multiway study of the temporal response of four yeast Saccharomyces cerevisiae strains with different glucose uptake rates upon altered metabolic conditions.
resultsWe measured the expression of 18 genes as function of time after addition of glucose to four strains of yeast grown in ethanol. The data are analyzed by matrix-augmented PCA, which is a generalization of PCA for 3-way data, and the results are confirmed by hierarchical clustering and clustering by Kohonen self-organizing map. Our approach identifies gene groups that respond similarly to the change of nutrient, and genes that behave differently in mutant strains. Of particular interest is our finding that ADH4 and ADH6 show a behavior typical of glucose-induced genes, while ADH3 and ADH5 are repressed after glucose addition.
conclusionMultiway real-time PCR gene expression profiling is a powerful technique which can be utilized to characterize functions of new genes by, for example, comparing their temporal response after perturbation in different genetic variants of the studied subject. The technique also identifies genes that show perturbed expression in specific strains.
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