ArticleBMC molecular biology2008
Drosophila Uri, a PP1alpha binding protein, is essential for viability, maintenance of DNA integrity and normal transcriptional activity.
Article in BMC molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 36 citations in OpenAlex.
- The Fascinating Intricacy of pSer/Thr-Specific Phosphatases and Their Higher-Order Complexes: Emerging Concepts.Biochemistry · 2025Review
- Maf1 phosphorylation is regulated through the action of prefoldin-like Bud27 on PP4 phosphatase in Saccharomyces cerevisiae.Nucleic acids research · 2024Article
- A strategy to disentangle direct and indirect effects on (de)phosphorylation by chemical modulators of the phosphatase PP1 in complex cellular contexts.Chemical science · 2024Article
- Review
- RMP promotes the proliferation and radioresistance of esophageal carcinoma.Journal of Cancer · 2019Article
- Ciliary dynein motor preassembly is regulated by Wdr92 in association with HSP90 co-chaperone, R2TP.The Journal of cell biology · 2018Article
- UXT is required for spermatogenesis in mice.PloS one · 2018Article
- Rpb5, a subunit shared by eukaryotic RNA polymerases, cooperates with prefoldin-like Bud27/URI.AIMS genetics · 2018Review
- URI Regulates KAP1 Phosphorylation and Transcriptional Repression via PP2A Phosphatase in Prostate Cancer Cells.The Journal of biological chemistry · 2016Article
- Heparan sulfate regulates the number and centrosome positioning of Drosophila male germline stem cells.Molecular biology of the cell · 2016Article
- URI prevents potassium dichromate-induced oxidative stress and cell death in gastric cancer cells.American journal of translational research · 2016Article
- The yeast prefoldin-like URI-orthologue Bud27 associates with the RSC nucleosome remodeler and modulates transcription.Nucleic acids research · 2014Article
- Drosophila Spag is the homolog of RNA polymerase II-associated protein 3 (RPAP3) and recruits the heat shock proteins 70 and 90 (Hsp70 and Hsp90) during the assembly of cellular machineries.The Journal of biological chemistry · 2014Article
- Upregulation of URI/RMP gene expression in cervical cancer by high-throughput tissue microarray analysis.International journal of clinical and experimental pathology · 2013Article
- Expression analysis of URI/RMP gene in endometrioid adenocarcinoma by tissue microarray immunohistochemistry.International journal of clinical and experimental pathology · 2013Article
- RMP plays distinct roles in the proliferation of hepatocellular carcinoma cells and normal hepatic cells.International journal of biological sciences · 2013Article
- The prefoldin bud27 mediates the assembly of the eukaryotic RNA polymerases in an rpb5-dependent manner.PLoS genetics · 2013Article
- Tissue, cell type and stage-specific ectopic gene expression and RNAi induction in the Drosophila testis.Spermatogenesis · 2012Article
- Regulation of androgen receptor-mediated transcription by RPB5 binding protein URI/RMP.Molecular and cellular biology · 2011Article
- RPB5-mediating protein is required for the proliferation of hepatocellular carcinoma cells.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundProtein phosphatase 1 (PP1) is involved in diverse cellular processes, and is targeted to substrates via interaction with many different protein binding partners. PP1 catalytic subunits (PP1c) fall into PP1alpha and PP1beta subfamilies based on sequence analysis, however very few PP1c binding proteins have been demonstrated to discriminate between PP1alpha and PP1beta.
resultsURI (unconventional prefoldin RPB5 interactor) is a conserved molecular chaperone implicated in a variety of cellular processes, including the transcriptional response to nutrient signalling and maintenance of DNA integrity. We show that Drosophila Uri binds PP1alpha with much higher affinity than PP1beta, and that this ability to discriminate between PP1c forms is conserved to humans. Most Uri is cytoplasmic, however we found some protein associated with active RNAPII on chromatin. We generated a uri loss of function allele, and show that uri is essential for viability in Drosophila. uri mutants have transcriptional defects, reduced cell viability and differentiation in the germline, and accumulate DNA damage in their nuclei.
conclusionUri is the first PP1alpha specific binding protein to be described in Drosophila. Uri protein plays a role in transcriptional regulation. Activity of uri is required to maintain DNA integrity and cell survival in normal development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.