ArticleBMC cell biology2008
The influence of nicotine on granulocytic differentiation - inhibition of the oxidative burst and bacterial killing and increased matrix metalloproteinase-9 release.
Article in BMC cell biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 52 citations in OpenAlex.
- Tobacco-enhanced biofilm formation by Porphyromonas gingivalis and other oral microbes.Molecular oral microbiology · 2024Review
- Outcomes of 576 patients with extracorporeal life support for the treatment of perioperative cardiogenic shock.Interdisciplinary cardiovascular and thoracic surgery · 2024Article
- A pleiotropic role of sialidase in the pathogenicity ofInfection and immunity · 2024Article
- Aerosolized nicotine from e-cigarettes alters gene expression, increases lung protein permeability, and impairs viral clearance in murine influenza infection.Frontiers in immunology · 2023Article
- Cigarette Smoke Exposure Promotes Virulence of Pseudomonas aeruginosa and Induces Resistance to Neutrophil Killing.Infection and immunity · 2020Article
- Tobacco-induced suppression of the vascular response to dental plaque.Molecular oral microbiology · 2018Review
- A Comparison between Two Pathophysiologically Different yet Microbiologically Similar Lung Diseases: Cystic Fibrosis and Chronic Obstructive Pulmonary Disease.International journal of respiratory and pulmonary medicine · 2018Article
- Granulocytes as models for human protein marker identification following nicotine exposure.Journal of neurochemistry · 2017Review
- The Protective Effect of Alpha 7 Nicotinic Acetylcholine Receptor Activation on Critical Illness and Its Mechanism.International journal of biological sciences · 2017Review
- A Case-Control Study of Comparison of Plasma Levels of Matrix Metalloproteinase-9 in Inhaled Opium Addicts and Clinically Healthy Persons.Addiction & health · 2017Article
- Nicotine induces neutrophil extracellular traps.Journal of leukocyte biology · 2016Article
- Acetylcholine Protects against Candida albicans Infection by Inhibiting Biofilm Formation and Promoting Hemocyte Function in a Galleria mellonella Infection Model.Eukaryotic cell · 2015Article
- Respiratory complications of organophosphorus nerve agent and insecticide poisoning. Implications for respiratory and critical care.American journal of respiratory and critical care medicine · 2014Review
- Chronic obstructive pulmonary disease (COPD): evaluation from clinical, immunological and bacterial pathogenesis perspectives.Journal of microbiology (Seoul, Korea) · 2014Review
- Smokeless tobacco use as a risk factor for periodontal disease.Frontiers in public health · 2014Article
- Neutrophils alter epithelial response to Porphyromonas gingivalis in a gingival crevice model.Molecular oral microbiology · 2013Article
- An update review on risk factors and scales for prediction of deep sternal wound infections.International wound journal · 2012Review
- Neutrophils in periodontal inflammation.Frontiers of oral biology · 2012Review
- Nicotinic receptor Alpha7 expression during tooth morphogenesis reveals functional pleiotropy.PloS one · 2012Article
- Cigarette smoke-exposed neutrophils die unconventionally but are rapidly phagocytosed by macrophages.Cell death & disease · 2011Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
Abstract
backgroundNeutrophils leave the bone marrow as terminally differentiated cells, yet little is known of the influence of nicotine or other tobacco smoke components on neutrophil differentiation. Therefore, promyelocytic HL-60 cells were differentiated into neutrophils using dimethylsulfoxide in the presence and absence of nicotine (3-(1-methyl-2-pyrrolidinyl) pyridine). Differentiation was evaluated over 5 days by monitoring terminal differentiation markers (CD11b expression and formazan deposition); cell viability, growth phase, kinetics, and apoptosis; assessing cellular morphology and ultrastructure; and conformational changes to major cellular components. Key neutrophil effector functions (oxidative burst, bacterial killing, matrix metalloproteinase release) were also examined.
resultsNicotine increased the percentage of cells in late differentiation phases (metamyelocytes, banded neutrophils and segmented neutrophils) compared to DMSO alone (p < 0.05), but did not affect any other marker of neutrophil differentiation examined. However, nicotine exposure during differentiation suppressed the oxidative burst in HL-60 cells (p < 0.001); inhibited bacterial killing (p < 0.01); and increased the LPS-induced release of MMP-9, but not MMP-2 (p < 0.05). These phenomena may be alpha-7-acetylcholine nicotinic receptor-dependent. Furthermore, smokers exhibited an increased MMP-9 burden compared to non-smokers in vivo (p < 0.05).
conclusionThese findings may partially explain the known increase in susceptibility to bacterial infection and neutrophil-associated destructive inflammatory diseases in individuals chronically exposed to nicotine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.