ArticleArthritis research & therapy2008
Analysis of bacterial DNA in synovial tissue of Tunisian patients with reactive and undifferentiated arthritis by broad-range PCR, cloning and sequencing.
Article in Arthritis research & therapy, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 49 citations in OpenAlex.
- Multinational evidence-based recommendations on how to investigate and follow-up undifferentiated peripheral inflammatory arthritis: integrating systematic literature research and expert opinion of a broad international panel of rheumatologists in the 3E Initiative.Annals of the rheumatic diseases · 2011Guideline
- Is Osteoarthritis a State of Joint Dysbiosis?Antibiotics (Basel, Switzerland) · 2025Review
- The first case of urosepsis caused byFuture microbiology · 2024Article
- Relationship between gut microbiota and rheumatoid arthritis: A bibliometric analysis.Frontiers in immunology · 2023Article
- Detection of bacterial DNA in synovial fluid in dogs with arthritis: a comparison between bacterial culture and 16S rRNA polymerase chain reaction.Acta veterinaria Scandinavica · 2021Article
- Uveitis in spondyloarthritis.Therapeutic advances in musculoskeletal disease · 2020Review
- Molecular characterisation of the synovial fluid microbiome in rheumatoid arthritis patients and healthy control subjects.PloS one · 2019Article
- The microbiome and HLA-B27-associated acute anterior uveitis.Nature reviews. Rheumatology · 2018Review
- Detection of Shigella spp. nucleic acids in the synovial tissue of Tunisian rheumatoid arthritis patients and other forms of arthritis by quantitative real-time polymerase chain reaction.Rheumatology international · 2018Article
- First-time detection and identification of the Mycobacterium tuberculosis Complex members in extrapulmonary tuberculosis clinical samples in south Tunisia by a single tube tetraplex real-time PCR assay.PLoS neglected tropical diseases · 2017Article
- Article
- Periodontal bacterial colonization in synovial tissues exacerbates collagen-induced arthritis in B10.RIII mice.Arthritis research & therapy · 2016Article
- [PCR-based detection of pathogens in clinical rheumatology].Zeitschrift fur Rheumatologie · 2016Review
- Gut inflammation and microbiome in spondyloarthritis.Rheumatology international · 2016Review
- Pneumonia and Pleural Empyema due to a MixedFrontiers in medicine · 2016Article
- Detection of Bacteria Bearing Resistant Biofilm Forms, by Using the Universal and Specific PCR is Still Unhelpful in the Diagnosis of Periprosthetic Joint Infections.Frontiers in medicine · 2014Article
- Article
- Broad-range PCR, cloning and sequencing of the full 16S rRNA gene for detection of bacterial DNA in synovial fluid samples of Tunisian patients with reactive and undifferentiated arthritis.Arthritis research & therapy · 2009Article
- A broad-range PCR technique for the diagnosis of infective endocarditis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
Corrections and comments
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Authors and funding
14 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionBacteria and/or their antigens have been implicated in the pathogenesis of reactive arthritis (ReA). Several studies have reported the presence of bacterial antigens and nucleic acids of bacteria other than those specified by diagnostic criteria for ReA in joint specimens from patients with ReA and various arthritides. The present study was conducted to detect any bacterial DNA and identify bacterial species that are present in the synovial tissue of Tunisian patients with reactive arthritis and undifferentiated arthritis (UA) using PCR, cloning and sequencing.
methodsWe examined synovial tissue samples from 28 patients: six patients with ReA and nine with UA, and a control group consisting of seven patients with rheumatoid arthritis and six with osteoarthritis (OA). Using broad-range bacterial PCR producing a 1,400-base-pair fragment from the 16S rRNA gene, at least 24 clones were sequenced for each synovial tissue sample. To identify the corresponding bacteria, DNA sequences were compared with sequences from the EMBL (European Molecular Biology Laboratory) database.
resultsBacterial DNA was detected in 75% of the 28 synovial tissue samples. DNA from 68 various bacterial species were found in ReA and UA samples, whereas DNA from 12 bacteria were detected in control group samples. Most of the bacterial DNAs detected were from skin or intestinal bacteria. DNA from bacteria known to trigger ReA, such as Shigella flexneri and Shigella sonnei, were detected in ReA and UA samples of synovial tissue and not in control samples. DNA from various bacterial species detected in this study have not previously been found in synovial samples.
conclusionThis study is the first to use broad-range PCR targeting the full 16S rRNA gene for detection of bacterial DNA in synovial tissue. We detected DNA from a wide spectrum of bacterial species, including those known to be involved in ReA and others not previously associated with ReA or related arthritis. The pathogenic significance of some of these intrasynovial bacterial DNAs remains unclear.
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