ArticleThe Journal of biological chemistry2008
The Legionella autoinducer synthase LqsA produces an alpha-hydroxyketone signaling molecule.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 120 citations in OpenAlex.
- Inter-kingdom signaling by the Legionella autoinducer LAI-1 involves the antimicrobial guanylate binding protein GBP.PLoS pathogens · 2025Article
- Review
- Small molecule communication ofMicrobiology and molecular biology reviews : MMBR · 2024Review
- Article
- Article
- Ecology ofBiofilm · 2024Review
- A Geneticist Transcribing the Chemical Language of Bacteria.Israel journal of chemistry · 2023Article
- Noncanonical Functions of Enzyme Cofactors as Building Blocks in Natural Product Biosynthesis.JACS Au · 2022Review
- Migration of Acanthamoeba through Legionella biofilms is regulated by the bacterial Lqs-LvbR network, effector proteins and the flagellum.Environmental microbiology · 2022Article
- TheApplied and environmental microbiology · 2022Article
- Genome Analysis of theCurrent issues in molecular biology · 2021Article
- Quorum sensing governs a transmissive Legionella subpopulation at the pathogen vacuole periphery.EMBO reports · 2021Article
- Legionella spp. All Ears? The Broad Occurrence of Quorum Sensing Elements outside Legionella pneumophila.Genome biology and evolution · 2021Article
- Quorum sensing controls persistence, resuscitation, and virulence of Legionella subpopulations in biofilms.The ISME journal · 2021Article
- Article
- Vibrio parahaemolyticus cqsA controls production of quorum sensing signal molecule 3-hydroxyundecan-4-one and regulates colony morphology.Journal of microbiology (Seoul, Korea) · 2019Article
- Quorum sensing modulates the formation of virulent Legionella persisters within infected cells.Nature communications · 2019Article
- Factors Mediating Environmental Biofilm Formation byFrontiers in cellular and infection microbiology · 2018Review
- Asymmetric regulation of quorum-sensing receptors drives autoinducer-specific gene expression programs in Vibrio cholerae.PLoS genetics · 2017Article
- Acyl Histidines: New N-Acyl Amides from Legionella pneumophila.Chembiochem : a European journal of chemical biology · 2017Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The opportunistic pathogen Legionella pneumophila replicates in human lung macrophages and in free-living amoebae. To accommodate the transfer between host cells, L. pneumophila switches from a replicative to a transmissive phase. L. pneumophila harbors a gene cluster homologous to the Vibrio cholerae cqsAS quorum sensing system, encoding a putative autoinducer synthase (lqsA) and a sensor kinase (lqsS), which flank a response regulator (lqsR). LqsR is an element of the L. pneumophila virulence regulatory network, which promotes pathogen-host cell interactions and inhibits entry into the replicative growth phase. Here, we show that lqsA functionally complements a V. cholerae cqsA autoinducer synthase deletion mutant and, upon expression in L. pneumophila or Escherichia coli, produces the diffusible signaling molecule LAI-1 (Legionella autoinducer-1). LAI-1 is distinct from CAI-1 (Cholerae autoinducer-1) and was identified as 3-hydroxypentadecan-4-one using liquid chromatography coupled to high resolution tandem mass spectrometry. The activity of both LqsA and CqsA was abolished upon mutation of a conserved lysine, and covalent binding of the cofactor pyridoxal 5'-phosphate to this lysine was confirmed by mass spectrometry. Thus, LqsA and CqsA belong to a family of pyridoxal 5'-phosphate-dependent autoinducer synthases, which produce the alpha-hydroxyketone signaling molecules LAI-1 and CAI-1.
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