ArticleNucleic acids research2008
Copper sensing function of Drosophila metal-responsive transcription factor-1 is mediated by a tetranuclear Cu(I) cluster.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
- EP300-Mediated MTF1-K218 Lactylation Buffers AR-Driven Copper Overload to Suppress Cuproptosis in Castration-Resistant Prostate Cancer.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Influence of metal ions on the economic parameters of mulberry silkworm Bombyx mori (Lepidoptera: Bombycidae).Biologia futura · 2025Article
- Emerging perspectives of copper-mediated transcriptional regulation in mammalian cell development.Metallomics : integrated biometal science · 2024Review
- Genomic Redistribution of Metal-Response Transcription Factor-1 (MTF-1) in Cadmium Resistant Cells.Cells · 2023Article
- Bioinformatics-Based Analysis of Key Genes in Steroid-Induced Osteonecrosis of the Femoral Head That Are Associated with Copper Metabolism.Biomedicines · 2023Article
- The Oncopig as an Emerging Model to Investigate Copper Regulation in Cancer.International journal of molecular sciences · 2022Review
- Primary nutrient sensors in plants.iScience · 2022Review
- The Hippo pathway kinases LATS1 and LATS2 attenuate cellular responses to heavy metals through phosphorylating MTF1.Nature cell biology · 2022Article
- Review
- The classic metal-sensing transcription factor MTF1 promotes myogenesis in response to copper.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Anatomy and Physiology of the Digestive Tract ofGenetics · 2018Review
- Metal-dependent allosteric activation and inhibition on the same molecular scaffold: the copper sensor CopY fromChemical science · 2018Article
- Copper and Zinc Homeostasis: Lessons fromFrontiers in genetics · 2017Review
- Bacterial cytosolic proteins with a high capacity for Cu(I) that protect against copper toxicity.Scientific reports · 2016Article
- Cellular sensing and transport of metal ions: implications in micronutrient homeostasis.The Journal of nutritional biochemistry · 2015Review
- Single nucleotide in the MTF-1 binding site can determine metal-specific transcription activation.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Efficient metal-specific transcription activation by Drosophila MTF-1 requires conserved cysteine residues in the carboxy-terminal domain.Biochimica et biophysica acta · 2012Article
- A novel role for copper in Ras/mitogen-activated protein kinase signaling.Molecular and cellular biology · 2012Article
- The CRR1 nutritional copper sensor in Chlamydomonas contains two distinct metal-responsive domains.The Plant cell · 2010Article
- Metalloproteins and metal sensing.Nature · 2009Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
Abstract
Drosophila melanogaster MTF-1 (dMTF-1) is a copper-responsive transcriptional activator that mediates resistance to Cu, as well as Zn and Cd. Here, we characterize a novel cysteine-rich domain which is crucial for sensing excess intracellular copper by dMTF-1. Transgenic flies expressing mutant dMTF-1 containing alanine substitutions of two, four or six cysteine residues within the sequence (547)CNCTNCKCDQTKSCHGGDC(565) are significantly or completely impaired in their ability to protect flies from copper toxicity and fail to up-regulate MtnA (metallothionein) expression in response to excess Cu. In contrast, these flies exhibit wild-type survival in response to copper deprivation thus revealing that the cysteine cluster domain is required only for sensing Cu load by dMTF-1. Parallel studies show that the isolated cysteine cluster domain is required to protect a copper-sensitive S. cerevisiae ace1Delta strain from copper toxicity. Cu(I) ligation by a Cys-rich domain peptide fragment drives the cooperative assembly of a polydentate [Cu(4)-S(6)] cage structure, characterized by a core of trigonally S(3) coordinated Cu(I) ions bound by bridging thiolate ligands. While reminiscent of Cu(4)-L(6) (L = ligand) tetranuclear clusters in copper regulatory transcription factors of yeast, the absence of significant sequence homology is consistent with convergent evolution of a sensing strategy particularly well suited for Cu(I).
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Registered trials
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