ArticleThe Plant cell1991
Monocot regulatory protein Opaque-2 is localized in the nucleus of maize endosperm and transformed tobacco plants.
Article in The Plant cell, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 62 citations in OpenAlex.
- Yeast-plant coupled vector system for identification of nuclear proteins.Plant physiology · 2007Article
- Wild-type opaque2 and defective opaque2 polypeptides form complexes in maize endosperm cells and bind the opaque2-zein target site.Plant physiology · 2007Article
- Interaction of maize Opaque-2 and the transcriptional co-activators GCN5 and ADA2, in the modulation of transcriptional activity.Plant molecular biology · 2004Article
- AtREM1, a member of a new family of B3 domain-containing genes, is preferentially expressed in reproductive meristems.Plant physiology · 2002Article
- Specific combinations of zein genes and genetic backgrounds influence the transcription of the heavy-chain zein genes in maize opaque-2 endosperms.Plant physiology · 2000Article
- The role of opaque2 in the control of lysine-degrading activities in developing maize endosperm.The Plant cell · 1999Article
- Molecular consequences of Ds insertion into and excision from the helix-loop-helix domain of the maize R gene.Genetics · 1998Article
- Phosphorylation of Opaque2 changes diurnally and impacts its DNA binding activity.The Plant cell · 1997Article
- A Ds insertion alters the nuclear localization of the maize transcriptional activator R.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Transcription factor veracity: is GBF3 responsible for ABA-regulated expression of Arabidopsis Adh?The Plant cell · 1996Article
- The conserved ELK-homeodomain of KNOTTED-1 contains two regions that signal nuclear localization.Plant molecular biology · 1996Article
- Nuclear localization signal binding proteins in higher plant nuclei.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- The rice bZIP transcriptional activator RITA-1 is highly expressed during seed development.The Plant cell · 1994Article
- Nuclear import of Agrobacterium VirD2 and VirE2 proteins in maize and tobacco.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Molecular analysis of wild-type and mutant alleles at the Opaque-2 regulatory locus of maize reveals different mutations and types of O2 products.Plant molecular biology · 1994Article
- Early auxin-induced genes encode short-lived nuclear proteins.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Functional expression of the transcriptional activator Opaque-2 of Zea mays in transformed yeast.Molecular & general genetics : MGG · 1993Article
- Specific binding of nuclear localization sequences to plant nuclei.The Plant cell · 1993Article
- Nuclear targeting of the maize R protein requires two nuclear localization sequences.Plant physiology · 1993Article
- Cytolocalization of zeatin O-xylosyltransferase in Phaseolus.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
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3 authors at 1 institution in 1 country.
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Abstract
Protein targeting to the nucleus has been studied extensively in animal and yeast systems; however, nothing is known about nuclear targeting in plants. The Opaque-2 (O2) gene produces a regulatory protein that is responsible for inducing transcription of the alpha-zein class of storage proteins in maize kernels. The cloned O2 gene encodes a protein that contains a leucine zipper DNA binding domain that can interact with zein gene promoters. We have used immunolocalization to show that the O2 protein is present in nuclei in the maize endosperm tissues known to produce alpha-zeins. In addition, neither embryo tissue from wild-type kernels nor endosperm from kernels harboring a null o2 allele contain the O2 protein. Analysis of a transposable, element-induced o2 allele, o2-m20, revealed that sectors of endosperm cells contained the nuclear-localized O2 protein, indicating excision of the transposable element. To study further the nuclear transport of the O2 protein, we have transformed this gene, under the control of a constitutive promoter, into tobacco. Plants were shown to have detectable levels of steady-state O2 mRNA and O2 protein. Immunolocalization of O2 protein in transformed tobacco plants indicated that the O2 protein was transported into tobacco nuclei. Therefore, we have developed a system to study nuclear targeting in plants and have established that the nuclear transport machinery is similar in monocots and dicots.
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