ArticleCurrent genetics2008
A codon-optimized luciferase from Gaussia princeps facilitates the in vivo monitoring of gene expression in the model alga Chlamydomonas reinhardtii.
Article in Current genetics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 118 citations in OpenAlex.
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- The Algal Chloroplast as a Testbed for Synthetic Biology Designs Aimed at Radically Rewiring Plant Metabolism.Frontiers in plant science · 2021Review
- An epigenetic gene silencing pathway selectively acting on transgenic DNA in the green alga Chlamydomonas.Nature communications · 2020Article
- Heterologous Gene Expression System Using the Cold-InducibleJournal of microbiology and biotechnology · 2020Article
- Validation of a New Multicistronic Plasmid for the Efficient and Stable Expression of Transgenes in Microalgae.International journal of molecular sciences · 2020Article
- Good News for Nuclear Transgene Expression inCells · 2019Review
- Cell wall integrity signaling regulates cell wall-related gene expression in Chlamydomonas reinhardtii.Scientific reports · 2019Article
- A Series of Fortunate Events: Introducing Chlamydomonas as a Reference Organism.The Plant cell · 2019Review
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- The Integration of Multiple Nuclear-Encoded Transgenes in the Green AlgaFrontiers in plant science · 2019Article
- Nourseothricin N-acetyl transferase (NAT), a new selectable marker for nuclear gene expression inPlant methods · 2019Article
- CITRIC: cold-inducible translational readthrough in the chloroplast of Chlamydomonas reinhardtii using a novel temperature-sensitive transfer RNA.Microbial cell factories · 2018Article
- Review
- Intron-containing algal transgenes mediate efficient recombinant gene expression in the green microalga Chlamydomonas reinhardtii.Nucleic acids research · 2018Article
- Identification and Functional Analysis of theInternational journal of molecular sciences · 2018Article
- Article
- Development and characterization of a novel luciferase based cytotoxicity assay.Scientific reports · 2018Article
- Using YFP as a Reporter of Gene Expression in the Green Alga Chlamydomonas reinhardtii.Methods in molecular biology (Clifton, N.J.) · 2018Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The unicellular green alga Chlamydomonas reinhardtii has emerged as a superb model species in plant biology. Although the alga is easily transformable, the low efficiency of transgene expression from the Chlamydomonas nuclear genome has severely hampered functional genomics research. For example, poor transgene expression is held responsible for the lack of sensitive reporter genes to monitor gene expression in vivo, analyze subcellular protein localization or study protein-protein interactions. Here, we have tested the luciferase from the marine copepod Gaussia princeps (G-Luc) for its suitability as a sensitive bioluminescent reporter of gene expression in Chlamydomonas. We show that a Gaussia luciferase gene variant, engineered to match the codon usage in the Chlamydomonas nuclear genome, serves as a highly sensitive reporter of gene expression from both constitutive and inducible algal promoters. Its bioluminescence signal intensity greatly surpasses previously developed reporters for Chlamydomonas nuclear gene expression and reaches values high enough for utilizing the reporter as a tool to monitor responses to environmental stresses in vivo and to conduct high-throughput screenings for signaling mutants in Chlamydomonas.
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